ATF5 antibody (Middle Region)

Catalog No. ABIN2787491

Product Details anti-ATF5 Antibody

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Zebrafish (Danio rerio), Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LPPPQQPPPP SPPQPSRLAP YPHPATTRGD RKQKKRDQNK SAALRYRQRK

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ATF5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ATF5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 282 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ATF5 antibody (ABIN2787491)

Wei, Jiang, Liu, Zhou, Chen, Zhang, Zong, Yun, Gu: "Cdc34-mediated degradation of ATF5 is blocked by cisplatin." in: The Journal of biological chemistry, Vol. 283, Issue 27, pp. 18773-81, (2008) (PubMed).

Target Details for ATF5

Target: ATF5 (Activating Transcription Factor 5 (ATF5))

Alternative Name: ATF5 (ATF5 Products)

Synonyms: ATF5 antibody, atfx antibody, hmfn0395 antibody, ATFX antibody, HMFN0395 antibody, AFTA antibody, Atf7 antibody, Atfx antibody, ODA-10 antibody, atf5 antibody, activating transcription factor 5 antibody, activating transcription factor 5, gene 2 S homeolog antibody, ATF5 antibody, atf5 antibody, Atf5 antibody, atf5.2.S antibody

Background: ATF5 belongs to the bZIP family. It is transcriptional activator which binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters and blocks the differentiation of neuroprogenitor cells into neurons. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Loss of ATF5 is associated with hepatocellular carcinoma.
Alias Symbols: ATFX, FLJ34666, HMFN0395
Protein Interaction Partner: CEBPE, CEBPA, GIT2, ATXN1, EP300, CCND3, GABBR2, DISC1, GABBR1, CEBPG, CDC34, TRIB3, UBC, CD34,
Protein Size: 282

Molecular Weight: 31 kDa

Gene ID: 22809

NCBI Accession: NM_012068, NP_036200

UniProt: B3KND3

Pathways: Myometrial Relaxation and Contraction