VPS52 antibody (Middle Region)

Catalog No. ABIN2787317

Product Details anti-VPS52 Antibody

Target: VPS52 (Vacuolar Protein Sorting 52 Homolog (VPS52))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS52 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RYWEQVLALL WPRFELILEM NVQSVRSTDP QRLGGLDTRP HYITRRYAEF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against VPS52. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human VPS52

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 723 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-VPS52 antibody (ABIN2787317)

Harrison, Haga, Pechenick Jowers, Jasim, Cintrat, Gillet, Schmitt-John, Digard, Beard: "Vaccinia virus uses retromer-independent cellular retrograde transport pathways to facilitate the wrapping of intracellular mature virions during viral morphogenesis." in: Journal of virology, (2016) (PubMed).

Target Details for VPS52

Target: VPS52 (Vacuolar Protein Sorting 52 Homolog (VPS52))

Alternative Name: VPS52 (VPS52 Products)

Synonyms: ARE1 antibody, RP5-1033B10 antibody, SAC2 antibody, SACM2L antibody, dJ1033B10.5 antibody, Are1 antibody, Sacm2l antibody, D130068D18 antibody, D430041K17Rik antibody, fb94c11 antibody, sacm2l antibody, wu:fb94c11 antibody, VPS52, GARP complex subunit antibody, VPS52 GARP complex subunit antibody, vacuolar protein sorting 52 homolog (S. cerevisiae) antibody, VPS52 antibody, Vps52 antibody, vps52 antibody

Background: This gene encodes a protein that is similar to the yeast suppressor of actin mutations 2 gene. The yeast protein forms a subunit of the tetrameric Golgi-associated retrograde protein complex that is involved in vesicle trafficking from from both early and late endosomes, back to the trans-Golgi network. This gene is located on chromosome 6 in a head-to-head orientation with the gene encoding ribosomal protein S18.
Alias Symbols: ARE1, DKFZp547I194, RP5-1033B10, SAC2, SACM2L, dJ1033B10.5
Protein Interaction Partner: FAM184A, MRPL11, SH2D4A, CCDC146, THAP11, KIAA1217, TBC1D22B, HEATR1, C1orf109, METTL13, ATP6V1D, VPS28, KANK2, TXN2, RNF41, EPM2AIP1, WTAP, USP2, STX11, LMO4, TRAF6, TPM3, TEAD4, RAB4A, PRKAA2, PRKAA1, NOP2, NFKBIB, MFAP1, KIF5B, GOLGA1, DDX6, AAMP, TXLN
Protein Size: 723

Molecular Weight: 82 kDa

Gene ID: 6293

NCBI Accession: NM_022553, NP_072047

UniProt: Q8N1B4