POLR1E antibody (N-Term)

Catalog No. ABIN2787316

Product Details anti-POLR1E Antibody

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR1E antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: SPGNMRFTLY ENKDSTNPRK RNQRILAAET DRLSYVGNNF GTGALKCNTL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against POLR1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLR1E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 419 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLR1E

Target: POLR1E (Polymerase (RNA) I Polypeptide E, 53kDa (POLR1E))

Alternative Name: POLR1E (POLR1E Products)

Synonyms: 53kDa antibody, AU042259 antibody, D030019D19Rik antibody, Paf53 antibody, Praf1 antibody, PAF53 antibody, PRAF1 antibody, RP11-405L18.3 antibody, RNA polymerase I subunit E antibody, polymerase (RNA) I polypeptide E antibody, polymerase (RNA) I polypeptide E L homeolog antibody, POLR1E antibody, polr1e antibody, Polr1e antibody, polr1e.L antibody

Background: POLR1E is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.POLR1E is the component of RNA polymerase I which synthesizes ribosomal RNA precursors.POLR1E appears to be involved in the formation of the initiation complex at the promoter by mediating the interaction between Pol I and UBTF/UBF.
Alias Symbols: FLJ13390, FLJ13970, PAF53, PRAF1, RP11-405L18.3
Protein Interaction Partner: UBC, RNF2, PAXIP1, CPSF3, ARRB1, CEBPA, SMARCAD1, RPA49, RRN3, POLR1A, SUPT16H, TAF1D, POLR1C, NFKBIB, POLR1B, TAF1C, UBTF, CD3EAP, QPCTL, DDX20, HNRNPUL1, DDX6,
Protein Size: 419

Molecular Weight: 47 kDa

Gene ID: 64425

NCBI Accession: NM_022490, NP_071935