PRAME antibody (N-Term)

Catalog No. ABIN2786465

Product Details anti-PRAME Antibody

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRAME antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR

Predicted Reactivity: Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 509 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRAME antibody (ABIN2786465)

Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." in: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, (2017) (PubMed).

Target Details for PRAME

Target: PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))

Alternative Name: PRAME (PRAME Products)

Synonyms: PRAME antibody, CT130 antibody, MAPE antibody, OIP-4 antibody, OIP4 antibody, 4930534P07Rik antibody, preferentially expressed antigen in melanoma antibody, PRAME antibody, Prame antibody

Background: PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Alias Symbols: MAPE, OIP4, CT130
Protein Interaction Partner: PRAME, RBX1, CUL2, TCEB2, TCEB1, ZNF408, FBXL12, XRN2, HIST4H4, HIST2H2BE, HIST2H2AC, HIST3H3, UBC, RARA, TP53RK, OSGEP, TPRKB, KDM1A, BAG2, LAGE3, UBL4A, SNRPB, RPS13, RPL22, RPL10, RFC4, PSMC4, HNRNPM, HNRNPH1, HNRNPF, HNRNPC, NEDD8, STK19,
Protein Size: 509

Molecular Weight: 56 kDa

Gene ID: 23532

NCBI Accession: NM_006115, NP_006106

UniProt: P78395

Pathways: Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding