SPAG8 antibody (Middle Region)
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- Target See all SPAG8 Antibodies
- SPAG8 (Sperm Associated Antigen 8 (SPAG8))
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Binding Specificity
- Middle Region
- Reactivity
- Human, Mouse, Rat, Rabbit, Cow, Dog, Pig
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SPAG8 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- PAPTKPHDYR QEQPETFWIQ RAPQLPTWWP LPTQVPAAED YLTWKEWGFT
- Predicted Reactivity
- Cow: 79%, Dog: 93%, Human: 100%, Mouse: 100%, Pig: 86%, Rabbit: 93%, Rat: 100%
- Characteristics
- This is a rabbit polyclonal antibody against SPAG8. It was validated on Western Blot using a cell lysate as a positive control.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide directed towards the middle region of human SPAG8
- Top Product
- Discover our top product SPAG8 Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 501 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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The M129V polymorphism of codon 129 in the prion gene (PRNP) in the Danish population." in: European journal of epidemiology, Vol. 23, Issue 1, pp. 23-7, (2008) (PubMed).
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The M129V polymorphism of codon 129 in the prion gene (PRNP) in the Danish population." in: European journal of epidemiology, Vol. 23, Issue 1, pp. 23-7, (2008) (PubMed).
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- Target
- SPAG8 (Sperm Associated Antigen 8 (SPAG8))
- Alternative Name
- SPAG8 (SPAG8 Products)
- Synonyms
- BS-84 antibody, HSD-1 antibody, SMP1 antibody, SPAG3 antibody, hSMP-1 antibody, MH-SPAG8 antibody, sperm associated antigen 8 antibody, SPAG8 antibody, Spag8 antibody
- Background
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The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. SPAG8 is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa.The correlation of anti-sperm antibodies with cases of unexplained infertility implicates a role for these antibodies in blocking fertilization. Improved diagnosis and treatment of immunologic infertility, as well as identification of proteins for targeted contraception, are dependent on the identification and characterization of relevant sperm antigens. The protein encoded by this gene is recognized by sperm agglutinating antibodies from an infertile woman. This protein is localized in germ cells of the testis at all stages of spermatogenesis and is localized to the acrosomal region of mature spermatozoa. Alternatively spliced variants that encode different protein isoforms have been described but the full-length sequences of only two have been determined.
Alias Symbols: BS-84, HSD-1, MGC26201, SMP1, SPAG3, hSMP-1
Protein Interaction Partner: KLHL32, DTX2, SNRPC, CSTF2, PITX1, JMJD4, WHSC1L1, KDM3B, CARM1, PRMT5, PRMT1, PRMT2, RANBP9, UBQLN4, NEDD4L, RAN,
Protein Size: 501 - Molecular Weight
- 53 kDa
- Gene ID
- 26206
- NCBI Accession
- NM_172312, NP_758516
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