FBXW8 antibody (Middle Region)

Catalog No. ABIN2786275

Product Details anti-FBXW8 Antibody

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Guinea Pig, Horse, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MNQKLWEVYS GHPVQHISFS SHSLITANVP YQTVMRNADL DSFTTHRRHR

Predicted Reactivity: Cow: 83%, Dog: 92%, Guinea Pig: 83%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 100%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against FBXW8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXW8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 598 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXW8 antibody (ABIN2786275)

Lin, Fu, Zhao, Lin, Zou, Liu, Zhu, Wang, Yu: "Fbxw8 is involved in the proliferation of human choriocarcinoma JEG-3 cells." in: Molecular biology reports, Vol. 38, Issue 3, pp. 1741-7, (2011) (PubMed).

Target Details for FBXW8

Target: FBXW8 (F-Box and WD Repeat Domain Containing 8 (FBXW8))

Alternative Name: FBXW8 (FBXW8 Products)

Synonyms: FBW6 antibody, FBW8 antibody, FBX29 antibody, FBXO29 antibody, FBXW6 antibody, 4930438M06Rik antibody, Fbx29 antibody, F-box and WD repeat domain containing 8 antibody, ring finger protein, transmembrane 2 antibody, F-box and WD-40 domain protein 8 antibody, FBXW8 antibody, Fbxw8 antibody, RNFT2 antibody

Background: FBXW8 is a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. FBXW8 contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class.This gene encodes a member of the F-box protein family, members of which are characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into three classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene contains a WD-40 domain, in addition to an F-box motif, so it belongs to the Fbw class. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Alias Symbols: FBW6, FBW8, FBX29, FBXO29, MGC33534, FBXW6
Protein Interaction Partner: MAP4K1, CUL1, TBC1D3, CUL7, UBE2D3, SKP1, IRS1, REST, CCND1, CUL4A, DDB1, UBC, CCDC8, GORASP1, OBSL1, CCT6B, CCT2, CCT7, TRIP13, PDCD5, CUL2, VBP1, CCT3, PFDN5, NFKBIA, CCT6A, BCL3, BTRC, SKP2, HSP90AA1, HSPA4, FBXO4, FBXW8, FBXW2, MYC, RBX1, UBI4, EP300,
Protein Size: 598

Molecular Weight: 67 kDa

Gene ID: 26259

NCBI Accession: NM_153348, NP_699179

UniProt: Q8N3Y1