ALKBH3 antibody (Middle Region)

Catalog No. ABIN2786204

Product Details anti-ALKBH3 Antibody

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Horse, Pig, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALKBH3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EMRKKPPPEE NGDYTYVERV KIPLDHGTLL IMEGATQADW QHRVPKEYHS

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against ALKBH3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ALKBH3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 286 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALKBH3 antibody (ABIN2786204)

Sundheim, Vågbø, Bjørås, Sousa, Talstad, Aas, Drabløs, Krokan, Tainer, Slupphaug: "Human ABH3 structure and key residues for oxidative demethylation to reverse DNA/RNA damage." in: The EMBO journal, Vol. 25, Issue 14, pp. 3389-97, (2006) (PubMed).

Target Details for ALKBH3

Target: ALKBH3 (AlkB, Alkylation Repair Homolog 3 (ALKBH3))

Alternative Name: ALKBH3 (ALKBH3 Products)

Synonyms: ABH3 antibody, DEPC-1 antibody, DEPC1 antibody, PCA1 antibody, 1700108H04Rik antibody, 1810020C19Rik antibody, Abh3 antibody, mABH3 antibody, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase L homeolog antibody, alkB homolog 3, alpha-ketoglutaratedependent dioxygenase antibody, alkB homolog 3, alpha-ketoglutarate-dependent dioxygenase antibody, alkbh3.L antibody, ALKBH3 antibody, Alkbh3 antibody

Background: The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine.The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 (MIM 610602) and ALKBH3 are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM]. Sequence Note: removed 1 base from the 5' end that did not align to the reference genome assembly. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1519 AB042029.1 2-1520
Alias Symbols: ABH3, DEPC-1, DEPC1, MGC118790, MGC118792, MGC118793, PCA1
Protein Interaction Partner: AK8, LNX1, GLRX3, IKZF1, GOLGA2, SRPK2, SRPK1,
Protein Size: 286

Molecular Weight: 33 kDa

Gene ID: 221120

NCBI Accession: NM_139178, NP_631917

UniProt: Q96Q83

Pathways: DNA Damage Repair