K-RAS antibody (N-Term)

Catalog No. ABIN2786191

Product Details anti-K-RAS Antibody

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This K-RAS antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: TEYKLVVVGA GGVGKSALTI QLIQNHFVDE YDPTIEDSYR KQVVIDGETC

Specificity: 100 % homologous to human KRAS, NRAS and HRAS.

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against KRAS. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human KRAS

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 189 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Alternative Name: KRAS (KRAS Products)

Target Type: Viral Protein

Background: KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.
Alias Symbols: C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS, NS3, RASK2
Protein Interaction Partner: RBPMS, UBC, PSMA3, ARAF, MDFI, TRAF6, BCL2, BTRC, RAF1, MAPK1, PHB, DDX50, EGLN1, DDX47, RALGDS, RGL2, PIK3R2, LPAR1, ADH6, APP, ELAVL1, CALM1, IGSF21, CCT7, PDE4DIP, DNAJA3, EEF1A1, RASGRF2, RASSF2, RAP1GDS1, RASGRP2, RASSF5, SHOC2, PGGT1B, PIK3CG, EPB42
Protein Size: 189

Molecular Weight: 22 kDa

Gene ID: 3845

NCBI Accession: NM_033360, NP_203524

UniProt: P01111