VPS4A antibody (N-Term)

Catalog No. ABIN2785950

Product Details anti-VPS4A Antibody

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Horse, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VPS4A antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: YFLHAIKYEA HSDKAKESIR AKCVQYLDRA EKLKDYLRSK EKHGKKPVKE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against VPS4A. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human VPS4A

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 437 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-VPS4A antibody (ABIN2785950)

Taylor, Hanson, Kielian: "Ubiquitin depletion and dominant-negative VPS4 inhibit rhabdovirus budding without affecting alphavirus budding." in: Journal of virology, Vol. 81, Issue 24, pp. 13631-9, (2007) (PubMed).

Target Details for VPS4A

Target: VPS4A (Vacuolar Protein Sorting-Associated Protein 4A (VPS4A))

Alternative Name: VPS4A (VPS4A Products)

Synonyms: vsp4 antibody, SKD1 antibody, SKD1A antibody, SKD2 antibody, VPS4 antibody, VPS4-1 antibody, zgc:153907 antibody, 4930589C15Rik antibody, AI325971 antibody, AW553189 antibody, vacuolar protein sorting 4 homolog A antibody, vacuolar sorting protein 4 antibody, vacuolar protein sorting 4 homolog A L homeolog antibody, vacuolar protein sorting 4a homolog A (S. cerevisiae) antibody, vacuolar protein sorting 4A antibody, VPS4A antibody, TP03_0351 antibody, vps4a antibody, vps4a.L antibody, Vps4a antibody

Background: VPS4A is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast.The protein encoded by this gene is a member of the AAA protein family (ATPases associated with diverse cellular activities), and is the homolog of the yeast Vps4 protein. In humans, two paralogs of the yeast protein have been identified. The former share a high degree of aa sequence similarity with each other, and also with yeast Vps4 and mouse Skd1 proteins. The mouse Skd1 (suppressor of K+ transport defect 1) has been shown to be really an yeast Vps4 ortholog. Functional studies indicate that both human paralogs associate with the endosomal compartments, and are involved in intracellular protein trafficking, similar to Vps4 protein in yeast. The gene encoding this paralog has been mapped to chromosome 16, the gene for the other resides on chromosome 18. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: FLJ22197, SKD1, SKD2, VPS4, VPS4-1, SKD1A
Protein Interaction Partner: FBXO6, UBC, CFTR, KCND3, CHMP4B, CHMP1B, CHMP4A, CHMP2A, VPS4A, CHMP1A, CHMP4C, gag, VTA1, CHMP3, CHMP5, HGS, NPC1, BABAM1, VPS4B, RND2,
Protein Size: 437

Molecular Weight: 49 kDa

Gene ID: 27183

NCBI Accession: NM_013245, NP_037377

UniProt: Q9UN37

Pathways: Microtubule Dynamics, CXCR4-mediated Signaling Events