INPP5B antibody (Middle Region)

Catalog No. ABIN2785875

Product Details anti-INPP5B Antibody

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This INPP5B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IHNGQVPCHF EFINKPDEES YCKQWLNANP SRGFLLPDSD VEIDLELFVN

Predicted Reactivity: Dog: 85%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 92%, Rabbit: 77%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against INPP5B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human INPP5B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 913 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-INPP5B antibody (ABIN2785875)

Tothova, Kollipara, Huntly, Lee, Castrillon, Cullen, McDowell, Lazo-Kallanian, Williams, Sears, Armstrong, Passegué, DePinho, Gilliland: "FoxOs are critical mediators of hematopoietic stem cell resistance to physiologic oxidative stress." in: Cell, Vol. 128, Issue 2, pp. 325-39, (2007) (PubMed).

Target Details for INPP5B

Target: INPP5B (Inositol Polyphosphate-5-Phosphatase, 75kDa (INPP5B))

Alternative Name: INPP5B (INPP5B Products)

Synonyms: 75kDa antibody, AW260155 antibody, INPP5P antibody, 5PTase antibody, inositol polyphosphate-5-phosphatase B antibody, type II inositol 1,4,5-trisphosphate 5-phosphatase-like antibody, inositol polyphosphate-5-phosphatase B L homeolog antibody, inositol polyphosphate 5-phosphatase OCRL-1 antibody, INPP5B antibody, LOC100224437 antibody, inpp5b.L antibody, LOC100159919 antibody, Inpp5b antibody

Background: Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). INPP5B is the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus.Cellular calcium signaling is controlled by the production of inositol phosphates (IPs) by phospholipase C in response to extracellular signals. The IP signaling molecules are inactivated by a family of inositol polyphosphate-5-phosphatases (5-phosphatases). This gene encodes the type II 5-phosphatase. The protein is localized to the cytosol and mitochondria, and associates with membranes through an isoprenyl modification near the C-terminus. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
Alias Symbols: 5PTase, MGC65156, MGC71303
Protein Interaction Partner: UBC, APP, SMURF1,
Protein Size: 913

Molecular Weight: 77 kDa

Gene ID: 3633

NCBI Accession: NM_005540, NP_005531

UniProt: P32019