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ETF1 antibody (N-Term)

ETF1 Reactivity: Human, Mouse, Rat, Guinea Pig, Zebrafish (Danio rerio), Horse, Cow, Dog, Rabbit, Saccharomyces cerevisiae WB, IHC Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2785859
  • Target See all ETF1 Antibodies
    ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
    Binding Specificity
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 16
    • 7
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat, Guinea Pig, Zebrafish (Danio rerio), Horse, Cow, Dog, Rabbit, Saccharomyces cerevisiae
    Host
    • 14
    • 3
    Rabbit
    Clonality
    • 14
    • 3
    Polyclonal
    Conjugate
    • 17
    This ETF1 antibody is un-conjugated
    Application
    • 14
    • 7
    • 7
    • 6
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC)
    Sequence
    ISLIIPPKDQ ISRVAKMLAD EFGTASNIKS RVNRLSVLGA ITSVQQRLKL
    Predicted Reactivity
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%
    Characteristics
    This is a rabbit polyclonal antibody against ETF1. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human ETF1
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    Discover our top product ETF1 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 437 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ETF1 (Eukaryotic Translation Termination Factor 1 (ETF1))
    Alternative Name
    ETF1 (ETF1 Products)
    Synonyms
    D5S1995 antibody, ERF antibody, ERF1 antibody, RF1 antibody, SUP45L1 antibody, TB3-1 antibody, AI463371 antibody, D6Ertd109e antibody, eRF1 antibody, zeh0057 antibody, hm:zeh0057 antibody, wu:fa19e07 antibody, wu:fi31f01 antibody, cl1 antibody, RRF1 antibody, DDBDRAFT_0188023 antibody, DDBDRAFT_0191343 antibody, DDB_0188023 antibody, DDB_0191343 antibody, CG5605 antibody, Dm0184 antibody, Dmel\\CG5605 antibody, Group J antibody, group J antibody, l(3)00103 antibody, l(3)05637 antibody, l(3)77ABb antibody, l(3)neo28 antibody, Eukaryotic release factor 1 antibody, eukaryotic translation termination factor 1 antibody, eukaryotic translation termination factor 1b antibody, eukaryotic translation termination factor 1 S homeolog antibody, eukaryotic peptide chain release factor subunit 1 antibody, eukaryotic petide chain release factor subunit 1 antibody, eukaryotic release factor 1 antibody, Eukaryotic peptide chain release factor subunit 1 antibody, ETF1 antibody, Etf1 antibody, etf1b antibody, etf1 antibody, etf1.S antibody, Tb11.22.0012 antibody, THAPSDRAFT_22240 antibody, TTHERM_00292170 antibody, LOC692937 antibody, erf1 antibody, eRF1 antibody, erfa-1 antibody
    Background
    Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA, however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).
    Alias Symbols: D5S1995, ERF, ERF1, MGC111066, RF1, SUP45L1, TB3-1
    Protein Interaction Partner: KEAP1, UBC, RTF1, NUDC, VPS26A, TSN, CORO1C, CORO1B, HECW2, ALB, UPF1, LIG4, GSPT1, PPP2CA, GSPT2, PABPC1,
    Protein Size: 437
    Molecular Weight
    49 kDa
    Gene ID
    2107
    NCBI Accession
    NM_004730, NP_004721
    UniProt
    P62495
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