GCLC antibody (Middle Region)

Catalog No. ABIN2785780

Product Details anti-GCLC Antibody

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Dog, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GCLC antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: RISKSRYDSI DSYLSKCGEK YNDIDLTIDK EIYEQLLQEG IDHLLAQHVA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against GCLC. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GCLC

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 637 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GCLC antibody (ABIN2785780)

Ramani, Tomasi, Yang, Ko, Lu: "Mechanism and significance of changes in glutamate-cysteine ligase expression during hepatic fibrogenesis." in: The Journal of biological chemistry, Vol. 287, Issue 43, pp. 36341-55, (2012) (PubMed).

Bardag-Gorce, Oliva, Lin, Li, French, French: "Proteasome inhibitor up regulates liver antioxidative enzymes in rat model of alcoholic liver disease." in: Experimental and molecular pathology, Vol. 90, Issue 1, pp. 123-30, (2011) (PubMed).

Target Details for GCLC

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: GCLC (GCLC Products)

Background: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. GCLC is the catalytic subunit of 637 amino acids with a calculated molecular weight of 72.773 kDa. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Alias Symbols: GCL, GCS, GLCL, GLCLC
Protein Interaction Partner: UBC, PPID, GCLM, CCL22, PAXIP1, ELAVL1,
Protein Size: 637

Molecular Weight: 73 kDa

Gene ID: 2729

NCBI Accession: NM_001498, NP_001489

UniProt: P48506

Pathways: Cell RedoxHomeostasis