GALE antibody (Middle Region)

Catalog No. ABIN2785691

Product Details anti-GALE Antibody

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Dog, Cow, Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GALE antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PQGIPNNLMP YVSQVAIGRR EALNVFGNDY DTEDGTGVRD YIHVVDLAKG

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against GALE. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human GALE

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 348 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for GALE

Target: GALE (UDP-Galactose-4-Epimerase (GALE))

Alternative Name: GALE (GALE Products)

Background: GALE is an UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in this gene result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and mental retardation, with symptoms ranging from mild ('peripheral' form) to severe ('generalized' form).
Alias Symbols: SDR1E1
Protein Interaction Partner: GALE, SUMO2, UBC, BAG3, FN1, APP,
Protein Size: 348

Molecular Weight: 38 kDa

Gene ID: 2582

NCBI Accession: NM_001008216, NP_001008217

UniProt: Q14376

Pathways: Response to Water Deprivation, Cellular Glucan Metabolic Process