ALAS1 antibody (N-Term)

Catalog No. ABIN2785663

Product Details anti-ALAS1 Antibody

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Sheep, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALAS1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: ETSAGPSVVS VKTDGGDPSG LLKNFQDIMQ KQRPERVSHL LQDNLPKSVS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 100%, Sheep: 93%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against ALAS1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ALAS1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 640 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ALAS1

Target: ALAS1 (Aminolevulinate, delta-, Synthase 1 (ALAS1))

Alternative Name: ALAS1 (ALAS1 Products)

Background: Delta-aminolevulinate synthase (ALAS, EC 2.3.1.37) catalyzes the condensation of glycine with succinyl-CoA to form delta-aminolevulinic acid. This nuclear-encoded mitochondrial enzyme is the first and rate-limiting enzyme in the mammalian heme biosynthetic pathway. There are 2 tissue-specific isozymes: a housekeeping enzyme encoded by the ALAS1 gene and an erythroid tissue-specific enzyme encoded by ALAS2.
Alias Symbols: ALAS, ALAS3, ALASH, MIG4
Protein Interaction Partner: FAM127C, KLHL35, ZNF564, TEKT4, DUSP19, SNX20, MTFR2, LONRF1, ZMAT1, CDC73, TTC23, CERK, SH2D4A, EP400, C2orf42, GNL3L, CCHCR1, POLDIP2, ZFYVE26, USP20, TMSB10, ZNF175, WIPF1, UBC, TMSB4XP6, TMSB4XP2, TMSB4XP1, TMSB4X, TCEA2, PPL, BCL7A, ALAS1, PLAU, VHL,
Protein Size: 640

Molecular Weight: 70 kDa

Gene ID: 211

NCBI Accession: NM_000688, NP_000679

UniProt: P13196

Pathways: Regulation of Lipid Metabolism by PPARalpha