FBXO31 antibody (Middle Region)

Catalog No. ABIN2785571

Product Details anti-FBXO31 Antibody

Target: FBXO31 (F-Box Protein 31 (FBXO31))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXO31 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VAAAEQPAQC GQGQPFVLPV GVSSRNEDYP RTCRMCFYGT GLIAGHGFTS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXO31. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXO31

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 367 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-FBXO31 antibody (ABIN2785571)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for FBXO31

Target: FBXO31 (F-Box Protein 31 (FBXO31))

Alternative Name: FBXO31 (FBXO31 Products)

Synonyms: 1110003O08Rik antibody, 2310046N15Rik antibody, Fbx14 antibody, Fbxo14 antibody, FBX14 antibody, FBXO14 antibody, Fbx31 antibody, pp2386 antibody, RGD1561069 antibody, fbx14 antibody, fbx31 antibody, fbxo14 antibody, fbxo31 antibody, F-box protein 31 antibody, F-box protein 31 S homeolog antibody, F-box protein 31 L homeolog antibody, Fbxo31 antibody, FBXO31 antibody, fbxo31 antibody, fbxo31.S antibody, fbxo31.L antibody

Background: FBXO31 is the component of some SCF (SKP1-cullin-F-box) protein ligase complex that plays a central role in G1 arrest following DNA damage. FBXO31 specifically recognizes phosphorylated cyclin-D1 (CCND1), promoting its ubiquitination and degradation by the proteasome, resulting in G1 arrest. FBXO31 may act as a tumor suppressor.Members of the F-box protein family, such as FBXO31, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Alias Symbols: DKFZP434B027, DKFZp434J1815, FBX14, FBXO14, FLJ22477, Fbx31, MGC15419, MGC9527, pp2386
Protein Interaction Partner: CDT1, UBE2L6, UBE2D1, CCND1, CUL1, MAP2K6, PARD6A, SKP1, COPS5, RBX1,
Protein Size: 367

Molecular Weight: 40 kDa

Gene ID: 79791

NCBI Accession: NM_024735, NP_079011

UniProt: Q5XUX0