MLF1 antibody (N-Term)

Catalog No. ABIN2785560

Product Details anti-MLF1 Antibody

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLF1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GRDLLSISDG RGRAHNRRGH NDGEDSLTHT DVSSFQTMDQ MVSNMRNYMQ

Predicted Reactivity: Human: 100%, Mouse: 79%, Rabbit: 90%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against MLF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MLF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 268 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MLF1 antibody (ABIN2785560)

Li, Wu, Jiang, Liu, Wu, Inagaki, Izawa, Mizisin, Engvall, Shelton: "Non-pathogenic protein aggregates in skeletal muscle in MLF1 transgenic mice." in: Journal of the neurological sciences, Vol. 264, Issue 1-2, pp. 77-86, (2007) (PubMed).

Target Details for MLF1

Target: MLF1 (Myeloid Leukemia Factor 1 (MLF1))

Alternative Name: MLF1 (MLF1 Products)

Background: MLF1 is involved in lineage commitment of primary hemopoietic progenitors by restricting erythroid formation and enhancing myeloid formation. It interferes with erythopoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 suppresses RFWD2/COP1 activity via CSN3 which activates p53 and induces cell cycle arrest.It binds DNA and affects the expression of a number of genes so may function as a transcription factor in the nucleus.
Alias Symbols: -
Protein Interaction Partner: CENPU, COPS3, UBC, NRBP1, YWHAZ, GRIK5, MAGEA11,
Protein Size: 268

Molecular Weight: 29 kDa

Gene ID: 4291

NCBI Accession: NM_022443, NP_071888

UniProt: P58340