AP2B1 antibody (C-Term)

Catalog No. ABIN2785455

Product Details anti-AP2B1 Antibody

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AP2B1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GAVDLLGGGL DSLLGSDLGG GIGGSPAVGQ SFIPSSVPAT FAPSPTPAVV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against AP2B1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human AP2B1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 951 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AP2B1 antibody (ABIN2785455)

Schmid, Ford, Burtey, Praefcke, Peak-Chew, Mills, Benmerah, McMahon: "Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly." in: PLoS biology, Vol. 4, Issue 9, pp. e262, (2006) (PubMed).

Target Details for AP2B1

Target: AP2B1 (Adaptor-Related Protein Complex 2, beta 1 Subunit (AP2B1))

Alternative Name: AP2B1 (AP2B1 Products)

Synonyms: 5-HT(2B) antibody, 5-HT2B antibody, ADTB2 antibody, AP105B antibody, AP2-BETA antibody, CLAPB1 antibody, 1300012O03Rik antibody, AI788979 antibody, fa16e07 antibody, wu:fa16c06 antibody, wu:fa16e07 antibody, wu:fc18c08 antibody, zgc:55659 antibody, 5-hydroxytryptamine receptor 2B antibody, adaptor related protein complex 2 beta 1 subunit antibody, adaptor-related protein complex 2, beta 1 subunit antibody, adaptor related protein complex 2 beta 1 subunit L homeolog antibody, HTR2B antibody, AP2B1 antibody, Ap2b1 antibody, ap2b1 antibody, ap2b1.L antibody

Background: AP2B1 is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. AP2B1 is found on the cytoplasmic face of coated vesicles in the plasma membrane.The protein encoded by this gene is one of two large chain components of the assembly protein complex 2, which serves to link clathrin to receptors in coated vesicles. The encoded protein is found on the cytoplasmic face of coated vesicles in the plasma membrane. Two transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: ADTB2, AP105B, AP2-BETA, CLAPB1, DKFZp781K0743
Protein Interaction Partner: NEU4, XRCC6BP1, THAP1, U2AF1, KPNA2, SLC25A6, AFF4, TXN2, PIP5K1C, RAPGEF3, AP1M2, SUMO2, GTF2I, UBC, EGFR, SUMO1, NEDD8, EPHA2, RPA3, RPA2, RPA1, SEC24C, NUSAP1, EPN1, XPO7, PLOD2, LNPEP, GRB2, LDLRAP1, AP2M1, UBD, RNF11, MMS19, ITGA4, FN1, ERBB2, DAB2,
Protein Size: 951

Molecular Weight: 105 kDa

Gene ID: 163

NCBI Accession: NM_001030006, NP_001025177

UniProt: P63010

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, EGFR Downregulation