LRRC50 antibody (N-Term)

Catalog No. ABIN2785360

Product Details anti-LRRC50 Antibody

Target: LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LRRC50 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LNDTLYLHFK GFDRIENLEE YTGLRCLWLQ SNGIQKIENL EAQTELRCLF

Predicted Reactivity: Cow: 93%, Dog: 93%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against LRRC50. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LRRC50

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 725 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-LRRC50 antibody (ABIN2785360)

Basten, Davis, Gillis, van Rooijen, Stoop, Babala, Logister, Heath, Jonges, Katsanis, Voest, van Eeden, Medema, Ketting, Schulte-Merker, Looijenga, Giles: "Mutations in LRRC50 predispose zebrafish and humans to seminomas." in: PLoS genetics, Vol. 9, Issue 4, pp. e1003384, (2013) (PubMed).

Yang, Zhou, Ma, Wang, Chen, Meng, Yu, Zhou, Sun: "Knockdown of PPAR δ gene promotes the growth of colon cancer and reduces the sensitivity to bevacizumab in nude mice model." in: PLoS ONE, Vol. 8, Issue 4, pp. e60715, (2013) (PubMed).

Lazrek, Goffard, Schanen, Karquel, Bocket, Lion, Devaux, Hedouin, Gosset, Hober: "Detection of hepatitis C virus antibodies and RNA among medicolegal autopsy cases in Northern France." in: Diagnostic microbiology and infectious disease, Vol. 55, Issue 1, pp. 55-8, (2006) (PubMed).

Target Details for LRRC50

Target: LRRC50 (Leucine Rich Repeat Containing 50 (LRRC50))

Alternative Name: LRRC50 (LRRC50 Products)

Background: LRRC50 contains 6 LRR (leucine-rich) repeats. It is proposed that LRRC50 to be a novel candidate gene for human cystic kidney disease, involved in regulation of microtubule-based cilia and actin-based brush border microvilli.
Alias Symbols: DKFZp434A119, FLJ25330, ODA7, CILD13, LRRC50
Protein Interaction Partner: UBC,
Protein Size: 725

Molecular Weight: 80 kDa

Gene ID: 123872

NCBI Accession: NM_178452, NP_848547

UniProt: Q8NEP3