CHMP4B antibody (Middle Region)

Catalog No. ABIN2785348

Product Details anti-CHMP4B Antibody

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHMP4B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: EEISTAISKP VGFGEEFDED ELMAELEELE QEELDKNLLE ISGPETVPLP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CHMP4B. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CHMP4B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 224 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHMP4B antibody (ABIN2785348)

Bodon, Chassefeyre, Pernet-Gallay, Martinelli, Effantin, Hulsik, Belly, Goldberg, Chatellard-Causse, Blot, Schoehn, Weissenhorn, Sadoul: "Charged multivesicular body protein 2B (CHMP2B) of the endosomal sorting complex required for transport-III (ESCRT-III) polymerizes into helical structures deforming the plasma membrane." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 40276-86, (2011) (PubMed).

Target Details for CHMP4B

Target: CHMP4B (Charged Multivesicular Body Protein 4B (CHMP4B))

Alternative Name: CHMP4B (CHMP4B Products)

Background: This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family. The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes. The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1. The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway. The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding. These polymers are proposed to help generate the luminal vesicles of multivesicular bodies. Mutations in this gene result in autosomal dominant posterior polar cataracts.
Alias Symbols: C20orf178, CHMP4A, CTPP3, SNF7, SNF7-2, Shax1, dJ553F4.4, VPS32B, Vps32-2
Protein Interaction Partner: UBC, TP53, CHMP6, UBE2I, PDCD6IP, VCAM1, ITGA4, RPLP0P6, MVP, ATP6V1B1, BROX, CHMP4B, CHMP4C, CHMP1B, CHMP3, CHMP5, CHMP4A, HIPK2, VPS4A, STAMBP, PIAS2, EXOSC9, HCVgp1, NOLC1, PTPN23,
Protein Size: 224

Molecular Weight: 25 kDa

Gene ID: 128866

NCBI Accession: NM_176812, NP_789782

UniProt: Q9H444