TTL antibody (Middle Region)

Catalog No. ABIN503450

Product Details anti-TTL Antibody

Target: TTL (Tubulin tyrosine Ligase (TTL))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TTL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LYREGVLRTA SEPYHVDNFQ DKTCHLTNHC IQKEYSKNYG KYEEGNEMFF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against TTL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human TTL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 377 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for TTL

Target: TTL (Tubulin tyrosine Ligase (TTL))

Alternative Name: TTL (TTL Products)

Background: TTL catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM].
Alias Symbols: MGC46235
Protein Interaction Partner: ELAVL1,
Protein Size: 377

Molecular Weight: 43 kDa

Gene ID: 150465

NCBI Accession: NM_153712, NP_714923

UniProt: Q8NG68

Pathways: Regulation of Cell Size