Crossover junction endonuclease EME1 (EME1) (Middle Region) antibody

Catalog No. ABIN503411

Product Details

Target: Crossover junction endonuclease EME1 (EME1)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Pig, Rabbit, Cow, Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Sequence: AYPSPQLLVQ AYQQCFSDKE RQNLLADIQV RRGEGVTSTS RRIGPELSRR

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 79%, Horse: 93%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against EME1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human EME1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 570 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details

Target: Crossover junction endonuclease EME1 (EME1)

Alternative Name: EME1 (EME1 Products)

Background: EME1 interacts with MUS81 to form a DNA structure-specific endonuclease with substrate preference for branched DNA structures with a 5'-end at the branch nick. Typical substrates include 3'-flap structures, replication forks and nicked Holliday junctions. EME1 may be required in mitosis for the processing of stalled or collapsed replication forks.EME1 and MUS81 (MIM 606591) form an endonuclease complex that cleaves branched DNA structures, especially those arising during stalled DNA replication (Abraham et al., 2003 [PubMed 14609959]).[supplied by OMIM].
Alias Symbols: FLJ31364, MMS4L, SLX2A
Protein Interaction Partner: vpr, MUS81, APP, SLX1A, EME1, SLX4, SUMO2, SLX1B,
Protein Size: 570

Molecular Weight: 63 kDa

Gene ID: 146956

NCBI Accession: NM_152463, NP_689676

UniProt: Q96AY2