FBXW10 antibody (Middle Region)

Catalog No. ABIN2784922

Product Details anti-FBXW10 Antibody

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Cow, Dog, Guinea Pig, Horse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FBXW10 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RKIHLLDIIQ VKAIPVEFRG HAGSVRALFL CEEENFLLSG SYDLSIRYWD

Predicted Reactivity: Cow: 100%, Dog: 93%, Guinea Pig: 77%, Horse: 86%, Human: 100%, Mouse: 100%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against FBXW10. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human FBXW10

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1051 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for FBXW10

Target: FBXW10 (F-Box and WD Repeat Domain Containing 10 (FBXW10))

Alternative Name: FBXW10 (FBXW10 Products)

Synonyms: Fbw10 antibody, HREP antibody, SM25H2 antibody, SM2SH2 antibody, CMT1A duplicated region transcript 1 antibody, F-box and WD repeat domain containing 10 antibody, tripartite motif containing 16 antibody, F-box and WD-40 domain protein 10 antibody, CDRT1 antibody, FBXW10 antibody, Fbxw10 antibody, Cdrt1 antibody, TRIM16 antibody

Background: Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.Members of the F-box protein family, such as FBXW10, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1, MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM].
Alias Symbols: Fbw10, HREP, SM25H2, SM2SH2
Protein Interaction Partner: CUL5,
Protein Size: 1051

Molecular Weight: 120 kDa

Gene ID: 10517

NCBI Accession: NM_031456, NP_113644

UniProt: Q5XX13