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ADD2 antibody (Middle Region)

ADD2 Reactivity: Human, Mouse, Rat, Cow, Horse, Pig, Dog, Rabbit, Guinea Pig WB, IF Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2784900
  • Target See all ADD2 Antibodies
    ADD2 (Adducin 2 (Beta) (ADD2))
    Binding Specificity
    • 15
    • 4
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    Human, Mouse, Rat, Cow, Horse, Pig, Dog, Rabbit, Guinea Pig
    Host
    • 41
    • 2
    Rabbit
    Clonality
    • 43
    Polyclonal
    Conjugate
    • 22
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This ADD2 antibody is un-conjugated
    Application
    • 35
    • 13
    • 13
    • 11
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF)
    Sequence
    VVNGREEEQT AEEILSKGLS QMTTSADTDV DTSKDKTESV TSGPMSPEGS
    Predicted Reactivity
    Cow: 100%, Dog: 100%, Guinea Pig: 86%, Horse: 100%, Human: 100%, Mouse: 79%, Pig: 100%, Rabbit: 93%, Rat: 100%
    Characteristics
    This is a rabbit polyclonal antibody against ADD2. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the middle region of human ADD2
    Top Product
    Discover our top product ADD2 Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 726 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    ADD2 (Adducin 2 (Beta) (ADD2))
    Alternative Name
    ADD2 (ADD2 Products)
    Synonyms
    si:dkey-202n14.5 antibody, ADDB antibody, 2900072M03Rik antibody, add97 antibody, beta-ADD antibody, adducin 2 antibody, adducin 2 (beta) antibody, ADD2 antibody, add2 antibody, Add2 antibody
    Background
    Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Adducins are heteromeric proteins composed of different subunits referred to as adducin alpha, beta and gamma. The three subunits are encoded by distinct genes and belong to a family of membrane skeletal proteins involved in the assembly of spectrin-actin network in erythrocytes and at sites of cell-cell contact in epithelial tissues. While adducins alpha and gamma are ubiquitously expressed, the expression of adducin beta is restricted to brain and hematopoietic tissues. Adducin, originally purified from human erythrocytes, was found to be a heterodimer of adducins alpha and beta. Polymorphisms resulting in amino acid substitutions in these two subunits have been associated with the regulation of blood pressure in an animal model of hypertension. Heterodimers consisting of alpha and gamma subunits have also been described. Structurally, each subunit is comprised of two distinct domains. The amino-terminal region is protease resistant and globular in shape, while the carboxy-terminal region is protease sensitive. The latter contains multiple phosphorylation sites for protein kinase C, the binding site for calmodulin, and is required for association with spectrin and actin. Various adducin beta mRNAs, alternatively spliced at 3'end and/or internally spliced and encoding different isoforms, have been described. The functions of all the different isoforms are not known.
    Alias Symbols: ADDB
    Protein Interaction Partner: UBC, APP, RPH3A, PRKACA, PRKCD, PTPRZ1, FYN, CALM1, ADD2, ADD1,
    Protein Size: 726
    Molecular Weight
    80 kDa
    Gene ID
    119
    NCBI Accession
    NM_001617, NP_001608
    UniProt
    P35612
    Pathways
    Regulation of Actin Filament Polymerization
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