POLI antibody (N-Term)

Catalog No. ABIN2784869

Product Details anti-POLI Antibody

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 740 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: POLI (POLI Products)

Synonyms: poli antibody, wu:fc27h11 antibody, zgc:110185 antibody, GB12093 antibody, NV16187 antibody, RAD30B antibody, RAD3OB antibody, Rad30b antibody, DNA polymerase iota antibody, polymerase (DNA directed) iota antibody, polymerase (DNA directed), iota antibody, POLI antibody, poli antibody, LOC551219 antibody, AFUA_4G11140 antibody, LOC5568276 antibody, Pol-iota antibody, Poli antibody

Background: POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
Protein Size: 740

Molecular Weight: 83 kDa

Gene ID: 11201

NCBI Accession: NM_007195, NP_009126

UniProt: Q9UNA4

Pathways: DNA Damage Repair