POLI antibody (N-Term)

Catalog No. ABIN2784869

Product Details anti-POLI Antibody

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Binding Specificity: N-Term

Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLI antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human POLI

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 740 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for POLI

Target: POLI (Polymerase (DNA Directed) iota (POLI))

Alternative Name: POLI (POLI Products)

Background: POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
Alias Symbols: RAD30B, RAD3OB
Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
Protein Size: 740

Molecular Weight: 83 kDa

Gene ID: 11201

NCBI Accession: NM_007195, NP_009126

UniProt: Q9UNA4

Pathways: DNA Damage Repair