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POLI antibody (N-Term)

POLI Reactivity: Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2784869
  • Target See all POLI Antibodies
    POLI (Polymerase (DNA Directed) iota (POLI))
    Binding Specificity
    • 15
    • 8
    • 8
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 40
    • 25
    • 10
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Horse, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Rabbit, Saccharomyces cerevisiae
    Host
    • 52
    • 3
    Rabbit
    Clonality
    • 52
    • 3
    Polyclonal
    Conjugate
    • 20
    • 5
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This POLI antibody is un-conjugated
    Application
    • 27
    • 27
    • 17
    • 13
    • 13
    • 6
    • 4
    • 4
    • 3
    • 3
    Western Blotting (WB)
    Sequence
    PVVERLGFDE NFVDLTEMVE KRLQQLQSDE LSAVTVSGHV YNNQSINLLD
    Predicted Reactivity
    Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 82%, Zebrafish: 86%
    Characteristics
    This is a rabbit polyclonal antibody against POLI. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human POLI
    Top Product
    Discover our top product POLI Primary Antibody
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 740 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    POLI (Polymerase (DNA Directed) iota (POLI))
    Alternative Name
    POLI (POLI Products)
    Synonyms
    poli antibody, wu:fc27h11 antibody, zgc:110185 antibody, GB12093 antibody, NV16187 antibody, RAD30B antibody, RAD3OB antibody, Rad30b antibody, DNA polymerase iota antibody, polymerase (DNA directed) iota antibody, polymerase (DNA directed), iota antibody, POLI antibody, poli antibody, LOC551219 antibody, AFUA_4G11140 antibody, LOC5568276 antibody, Pol-iota antibody, Poli antibody
    Background
    POLI is an error-prone DNA polymerase specifically involved in DNA repair.POLI plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.POLI favors Hoogsteen base-pairing in the active site.POLI inserts the correct base with high-fidelity opposite an adenosine template.POLI exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine.POLI may play a role in hypermutation of immunogobulin genes.POLI forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
    Alias Symbols: RAD30B, RAD3OB
    Protein Interaction Partner: TMEM239, TRIM39, ZBTB44, UBC, TRAF6, DAZAP2, RNF11, TRIM23, POLH, PCNA, APP, REV1, XRCC1, UBB, UBA52, CALCOCO2, DVL2,
    Protein Size: 740
    Molecular Weight
    83 kDa
    Gene ID
    11201
    NCBI Accession
    NM_007195, NP_009126
    UniProt
    Q9UNA4
    Pathways
    DNA Damage Repair
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