CLK1 antibody (N-Term)

Catalog No. ABIN2784736

Product Details anti-CLK1 Antibody

Target: CLK1 (CDC like Kinase 1 (CLK1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Horse, Rabbit, Rat, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLK1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: HSSAQENKRC KYNHSKMCDS HYLESRSINE KDYHSRRYID EYRNDYTQGC

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 86%, Rabbit: 100%, Rat: 85%

Characteristics: This is a rabbit polyclonal antibody against CLK1. It was validated on Western Blot.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CLK1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 484 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CLK1 antibody (ABIN2784736)

Aubol, Wu, Keshwani, Movassat, Fattet, Hertel, Fu, Adams: "Release of SR Proteins from CLK1 by SRPK1: A Symbiotic Kinase System for Phosphorylation Control of Pre-mRNA Splicing." in: Molecular cell, Vol. 63, Issue 2, pp. 218-228, (2016) (PubMed).

Eisenreich, Zakrzewicz, Huber, Thierbach, Pepke, Goldin-Lang, Schultheiss, Pries, Rauch: "Regulation of pro-angiogenic tissue factor expression in hypoxia-induced human lung cancer cells." in: Oncology reports, Vol. 30, Issue 1, pp. 462-70, (2013) (PubMed).

Target Details for CLK1

Target: CLK1 (CDC like Kinase 1 (CLK1))

Alternative Name: CLK1 (CLK1 Products)

Synonyms: CLK antibody, CLK/STY antibody, STY antibody, CDC like kinase 1 antibody, CDC-like kinase 1 antibody, CLK1 antibody, Clk1 antibody

Background: This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CLK, CLK/STY, STY
Protein Interaction Partner: KRTAP10-7, PDE9A, PRMT5, SRPK2, SRPK1, CLK1, BAG3, PIAS4, ISG15, UBC, YWHAG, LUC7L3, PPIG, SRSF6, PRPF4, GOLM1, UBL5, SRSF1, PTPN1, MBP, BCLAF1, NCOR1, SRSF3, SAFB,
Protein Size: 484

Molecular Weight: 57 kDa

Gene ID: 1195

NCBI Accession: NM_004071, NP_004062

UniProt: P49759