DBT antibody (N-Term)

Catalog No. ABIN2784728

Product Details anti-DBT Antibody

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DBT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: NYVCFFGYPS FKYSHPHHFL KTTAALRGQV VQFKLSDIGE GIREVTVKEW

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 83%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against DBT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human DBT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 482 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for DBT

Target: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Alternative Name: DBT (DBT Products)

Synonyms: BCATE2 antibody, BCKAD-E2 antibody, BCKADE2 antibody, E2 antibody, E2B antibody, D3Wsu60e antibody, im:7147214 antibody, zgc:103768 antibody, E2b antibody, dihydrolipoamide branched chain transacylase E2 antibody, hypothetical protein antibody, dihydrolipoamide branched chain transacylase E2 L homeolog antibody, DBT antibody, dbt antibody, CpipJ_CPIJ006326 antibody, BDBG_05874 antibody, NAEGRDRAFT_78509 antibody, VDBG_04820 antibody, PGTG_17722 antibody, Dbt antibody, dbt.L antibody

Target Type: Viral Protein

Background: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO2. It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3).The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: BCATE2, E2, E2B, MGC9061
Protein Interaction Partner: HUWE1, UBC, PARK2, IFIT2, ABCC1, DLD, AGO4, AGO3, SRRM2, USP16, ADI1,
Protein Size: 482

Molecular Weight: 46 kDa

Gene ID: 1629

NCBI Accession: NM_001918, NP_001909

UniProt: P11182