CK1 epsilon antibody (N-Term)

Catalog No. ABIN2784724

Product Details anti-CK1 epsilon Antibody

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Sheep, Rabbit, Guinea Pig, Horse, Cow, Saccharomyces cerevisiae, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MELRVGNKYR LGRKIGSGSF GDIYLGANIA SGEEVAIKLE CVKTKHPQLH

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast: 93%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against CSNK1E. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CSNK1E

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 416 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CK1 epsilon antibody (ABIN2784724)

Levran, Londono, OHara, Nielsen, Peles, Rotrosen, Casadonte, Linzy, Randesi, Ott, Adelson, Kreek: "Genetic susceptibility to heroin addiction: a candidate gene association study." in: Genes, brain, and behavior, Vol. 7, Issue 7, pp. 720-9, (2009) (PubMed).

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: CSNK1E (CSNK1E Products)

Background: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. CSNK1E can phosphorylate a large number of proteins. CSNK1E participates in Wnt signaling and is the central component of the circadian clock. It may act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. CSNK1E inhibits cytokine-induced granuloytic differentiation.The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.
Alias Symbols: HCKIE, MGC10398, CKIepsilon
Protein Interaction Partner: DVL3, TIAM1, UBC, REST, ZSWIM8, TAZ, SAV1, DVL2, ADAM22, SNX24, AHCYL2, PER1, MCC, HMMR, TCEB2, STK3, SEC13, CSNK1D, CSNK1A1, CRY1, ACACA, FAM83H, FAM83B, VPS13B, FAM199X, KLHDC3, FAM110B, FAM110A, FAM83D, C2orf44, SNX22, STOX2, UNC45A, PPP6R3, WDR5, SDCC
Protein Size: 416

Molecular Weight: 47 kDa

Gene ID: 1454

NCBI Accession: NM_001894, NP_001885

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase