SIN3B antibody (Middle Region)

Catalog No. ABIN2784242

Product Details anti-SIN3B Antibody

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Horse, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIN3B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: NDTKALRSKS LLNEIESVYD EHQEQHSEGR SAPSSEPHLI FVYEDRQILE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against SIN3B. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SIN3B

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1162 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SIN3B antibody (ABIN2784242)

Dhanda, Lindberg, Olsson: "The human SIN3B corepressor forms a nucleolar complex with leukemia-associated ETO homologues." in: BMC molecular biology, Vol. 9, pp. 8, (2008) (PubMed).

Target Details for SIN3B

Target: SIN3B (SIN3 Transcription Regulator Homolog B (SIN3B))

Alternative Name: SIN3B (SIN3B Products)

Synonyms: fj63d08 antibody, si:dkey-6n6.6 antibody, wu:fj63d08 antibody, 2810430C10Rik antibody, SIN3 transcription regulator family member B antibody, SIN3 transcription regulator family member B S homeolog antibody, transcriptional regulator, SIN3B (yeast) antibody, sin3b antibody, SIN3B antibody, sin3b.S antibody, Sin3b antibody

Background: SIN3B acts as a transcriptional repressor. It interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities.It also interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. SIN3B also forms a complex with FOXK1 which represses transcription.
Alias Symbols: KIAA0700
Protein Interaction Partner: SOX2, HDAC1, HDAC2, UBC, ESR1, AR, TFAP4, TP53, SUMO2, KLF16, MORF4L1, MORF4L2, MRGBP, KDM5A, CIITA, NR1D2, COPS2, SMN1, PBX1, REST, ZBTB4, ARID4A, MYT1, MXI1, SAP25, SMARCE1, SMARCB1, SMARCA4, SMARCA2, CBFA2T3, RNF220, ZBTB16, SETDB1, HDAC9, IKZF4, IKZF1
Protein Size: 1162

Molecular Weight: 133 kDa

Gene ID: 23309

NCBI Accession: NM_015260, NP_056075

UniProt: O75182