UBA3 antibody (N-Term)

Catalog No. ABIN2784205

Product Details anti-UBA3 Antibody

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Zebrafish (Danio rerio), Cow, Dog, Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: EGRWNHVKKF LERSGPFTHP DFEPSTESLQ FLLDTCKVLV IGAGGLGCEL

Predicted Reactivity: Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%

Characteristics: This is a rabbit polyclonal antibody against UBA3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human UBA3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 463 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for UBA3

Target: UBA3 (Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3))

Alternative Name: UBA3 (UBA3 Products)

Synonyms: UBE1C antibody, hUBA3 antibody, Ube1c antibody, ube1c antibody, wu:fb75e04 antibody, wu:fc37b11 antibody, zgc:55528 antibody, A830034N06Rik antibody, AI256736 antibody, AI848246 antibody, AW546539 antibody, ubiquitin like modifier activating enzyme 3 antibody, ubiquitin-like modifier activating enzyme 3 antibody, ubiquitin like modifier activating enzyme 3 S homeolog antibody, UBA3 antibody, Uba3 antibody, uba3 antibody, uba3.S antibody

Background: UBA3 is a catalytic subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. UBA3 down-regulates steroid receptor activity. UBA3 is necessary for cell cycle progression.The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: DKFZp566J164, MGC22384, UBE1C, hUBA3
Protein Interaction Partner: UBA3, UBC, IMPA2, CPNE2, NEDD8, CAMK1D, NSFL1C, DCPS, KDM1A, PAPSS1, NAE1, XPO1, PCYT2, CAPN2, AHCY, ACAT2, NT5DC1, NUDCD2, TXNDC17, WDR61, UFC1, DSTN, VCL, UBE2H, PRDX2, TWF1, UBE2M, UIMC1, CHD3,
Protein Size: 463

Molecular Weight: 52 kDa

Gene ID: 9039

NCBI Accession: NM_003968, NP_003959

UniProt: Q8TBC4