EIF3H antibody (N-Term)

Catalog No. ABIN2784203

Product Details anti-EIF3H Antibody

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Horse, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF3H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MASRKEGTGS TATSSSSTAG AAGKGKGKGG SGDSAVKQVQ IDGLVVLKII

Predicted Reactivity: Cow: 93%, Dog: 86%, Guinea Pig: 86%, Horse: 86%, Human: 100%, Mouse: 100%, Pig: 93%, Rabbit: 86%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against EIF3H. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human EIF3H

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 352 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-EIF3H antibody (ABIN2784203)

Ewing, Chu, Elisma, Li, Taylor, Climie, McBroom-Cerajewski, Robinson, OConnor, Li, Taylor, Dharsee, Ho, Heilbut, Moore, Zhang, Ornatsky, Bukhman, Ethier, Sheng, Vasilescu, Abu-Farha, Lambert, Duewel et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, (2007) (PubMed).

Target Details for EIF3H

Target: EIF3H (Eukaryotic Translation Initiation Factor 3 Subunit H (EIF3H))

Alternative Name: EIF3H (EIF3H Products)

Background: EIF3H belongs to the peptidase M67 family. It contains 1 MPN (JAB/Mov34) domain. EIF3H binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. It associates with the p170 subunit of EIF3.
Alias Symbols: EIF3S3, MGC102958, eIF3-gamma, eIF3-p40
Protein Interaction Partner: NCK2, UBC, ABI3, SORBS3, ABI2, YTHDF2, RPS20, RPS9, HECW2, TARDBP, EIF3D, NPM1, ICAM1, CSNK2A2, CD81, IGSF8, PAN2, gag-pol, EIF3B, HSP90AB1, HSP90AA1, DDX3X, SMURF1, EIF3E, EIF3L, PAXIP1, EIF3C, EIF3A, EIF3K, EIF3M, EIF3I, EIF3G, EIF3F, RPS6KB1, CDK2, YWH
Protein Size: 352

Molecular Weight: 40 kDa

Gene ID: 8667

NCBI Accession: NM_003756, NP_003747

UniProt: O15372

Pathways: Ribonucleoprotein Complex Subunit Organization