RFT1 antibody (N-Term)

Catalog No. ABIN2784093

Product Details anti-RFT1 Antibody

Target: RFT1 (RFT1 Homolog (RFT1))

Binding Specificity: N-Term

Reactivity: Human, Dog, Mouse, Cow, Guinea Pig, Horse, Rabbit, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RFT1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: GTQRDWSQTL NLLWLTVPLG VFWSLFLGWI WLQLLEVPDP NVVPHYATGV

Predicted Reactivity: Cow: 92%, Dog: 100%, Guinea Pig: 92%, Horse: 92%, Human: 100%, Mouse: 85%, Rabbit: 92%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against RFT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RFT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 541 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RFT1

Target: RFT1 (RFT1 Homolog (RFT1))

Alternative Name: RFT1 (RFT1 Products)

Background: N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a!!Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation.N-glycosylation of proteins follows a highly conserved pathway that begins with the synthesis of a Man(5)GlcNAc(2)-dolichylpyrophosphate (PP-Dol) intermediate on the cytoplasmic side of the endoplasmic reticulum (ER) membrane followed by the translocation of Man(5)GlcNAc (2)-PP-Dol to the luminal side of the ER membrane. RFT1 is the flippase enzyme that catalyzes this translocation (Helenius et al., 2002 [PubMed 11807558]).[supplied by OMIM].
Alias Symbols: CDG1N
Protein Interaction Partner: PSMA3, UBC, NEDD4,
Protein Size: 541

Molecular Weight: 60 kDa

Gene ID: 91869

NCBI Accession: NM_052859, NP_443091

UniProt: Q96AA3