SMPD3 antibody (Middle Region)

Catalog No. ABIN2783713

Product Details anti-SMPD3 Antibody

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD3 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RPPEADDPVP GGQARNGAGG GPRGQTPNHN QQDGDSGSLG SPSASRESLV

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SMPD3. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human SMPD3

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 655 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SMPD3 antibody (ABIN2783713)

Tani, Hannun: "Analysis of membrane topology of neutral sphingomyelinase 2." in: FEBS letters, Vol. 581, Issue 7, pp. 1323-8, (2007) (PubMed).

Target Details for SMPD3

Target: SMPD3 (Sphingomyelin Phosphodiesterase 3 (SMPD3))

Alternative Name: SMPD3 (SMPD3 Products)

Synonyms: 4631433G07Rik antibody, AI427456 antibody, AW537966 antibody, fro antibody, nSMase2 antibody, cca1 antibody, NSMASE2 antibody, sphingomyelin phosphodiesterase 3 antibody, sphingomyelin phosphodiesterase 3, neutral antibody, SMPD3 antibody, Smpd3 antibody

Background: SMPD3 catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. SMPD3 regulates the cell cycle by acting as a growth suppressor in confluent cells. It probably acts as a regulator of postnatal development and participates in bone and dentin mineralization.
Alias Symbols: FLJ22593, MGC138443, NSMASE2
Protein Size: 655

Molecular Weight: 71 kDa

Gene ID: 55512

NCBI Accession: NM_018667, NP_061137

UniProt: Q9NY59

Pathways: Hormone Transport