OGT antibody (N-Term)

Catalog No. ABIN2783662

Product Details anti-OGT Antibody

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Guinea Pig, Horse, Rabbit, Cow

Host: Rabbit

Clonality: Polyclonal

Conjugate: This OGT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: ASSVGNVADS TEPTKRMLSF QGLAELAHRE YQAGDFEAAE RHCMQLWRQE

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against OGT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human OGT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1046 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-OGT antibody (ABIN2783662)

Taylor, Parker, Hazel, Soesanto, Fuller, Yazzie, McClain: "Glucose deprivation stimulates O-GlcNAc modification of proteins through up-regulation of O-linked N-acetylglucosaminyltransferase." in: The Journal of biological chemistry, Vol. 283, Issue 10, pp. 6050-7, (2008) (PubMed).

Target Details for OGT

Target: OGT (O-Linked N-Acetylglucosamine (GlcNAc) Transferase (UDP-N-Acetylglucosamine:polypeptide-N-Acetylglucosaminyl Transferase) (OGT))

Alternative Name: OGT (OGT Products)

Background: OGT catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) catalyzes the addition of a single N-acetylglucosamine in O-glycosidic linkage to serine or threonine residues. Since both phosphorylation and glycosylation compete for similar serine or threonine residues, the two processes may compete for sites, or they may alter the substrate specificity of nearby sites by steric or electrostatic effects. The protein contains nine tetratricopeptide repeats and a putative bipartite nuclear localization signal. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Alias Symbols: FLJ23071, HRNT1, MGC22921, O-GLCNAC
Protein Interaction Partner: FBXW11, HUWE1, UBC, PSG1, PHC3, NUP62CL, TET2, SUMO1, NEDD8, WWOX, BAP1, P3H1, RPRD1B, PPME1, LAP3, DBNL, HSPBP1, GPN1, WDR4, ACTR2, TOM1L1, ZPR1, SURF2, LPP, AGFG1, ALAD, C14orf142, UBL4A, FOXO4, HCFC1, SMURF1, Hoxa1, Nfatc1, Nup62, RELA, PPP1CB, PPP1CA,
Protein Size: 1046

Molecular Weight: 117 kDa

Gene ID: 8473

NCBI Accession: NM_181672, NP_858058

UniProt: O15294

Pathways: Regulation of Carbohydrate Metabolic Process