ALG2 antibody (C-Term)

Catalog No. ABIN2783587

Product Details anti-ALG2 Antibody

Target: ALG2 (Asparagine-Linked Glycosylation 2, alpha-1,3-Mannosyltransferase Homolog (ALG2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae, Goat, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ALG2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QSDLGQYVTF LRSFSDKQKI SLLHSCTCVL YTPSNEHFGI VPLEAMYMQC

Predicted Reactivity: Cow: 100%, Dog: 93%, Goat: 86%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against ALG2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human ALG2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 416 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ALG2 antibody (ABIN2783587)

Cossins, Belaya, Hicks, Salih, Finlayson, Carboni, Liu, Maxwell, Zoltowska, Farsani, Laval, Seidhamed, Donnelly, Bentley, McGowan, Müller, Palace, Lochmüller, Beeson: "Congenital myasthenic syndromes due to mutations in ALG2 and ALG14." in: Brain : a journal of neurology, Vol. 136, Issue Pt 3, pp. 944-56, (2013) (PubMed).

Rind, Schmeiser, Thiel, Absmanner, Lübbehusen, Hocks, Apeshiotis, Wilichowski, Lehle, Körner: "A severe human metabolic disease caused by deficiency of the endoplasmatic mannosyltransferase hALG11 leads to congenital disorder of glycosylation-Ip." in: Human molecular genetics, Vol. 19, Issue 8, pp. 1413-24, (2010) (PubMed).

Target Details for ALG2

Target: ALG2 (Asparagine-Linked Glycosylation 2, alpha-1,3-Mannosyltransferase Homolog (ALG2))

Alternative Name: ALG2 (ALG2 Products)

Synonyms: CDGIi antibody, NET38 antibody, hALPG2 antibody, 1110018A23Rik antibody, 1300013N08Rik antibody, ALPG2 antibody, MNCb-5081 antibody, im:7145131 antibody, ALG2, alpha-1,3/1,6-mannosyltransferase antibody, asparagine-linked glycosylation 2 (alpha-1,3-mannosyltransferase) antibody, ALG2, alpha-1,3/1,6-mannosyltransferase L homeolog antibody, GDP-Man:Man(1)GlcNAc(2)-PP-dolichol alpha-1,3-mannosyltransferase antibody, ALG2 antibody, Alg2 antibody, alg2.L antibody, alg2 antibody

Background: ALG2 is a member of the glycosyltransferase 1 family. It acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii).This gene encodes a member of the glycosyltransferase 1 family. The encoded protein acts as an alpha 1,3 mannosyltransferase, mannosylating Man(2)GlcNAc(2)-dolichol diphosphate and Man(1)GlcNAc(2)-dolichol diphosphate to form Man(3)GlcNAc(2)-dolichol diphosphate. Defects in this gene have been associated with congenital disorder of glycosylation type Ih (CDG-Ii). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: CDGIi, FLJ14511, hALPG2, NET38
Protein Interaction Partner: UBC, PLSCR3, SHISA5, PDCD6IP, ALG2, VPS28, PTPN23, SEC31A, TSG101, LAMP1, HSP90AA1, PTK2B, ANXA11, ANXA7, VPS37A, PEF1, SRI,
Protein Size: 416

Molecular Weight: 47 kDa

Gene ID: 85365

NCBI Accession: NM_033087, NP_149078

UniProt: Q9H553