NSUN2 antibody (C-Term)

Catalog No. ABIN2783512

Product Details anti-NSUN2 Antibody

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSUN2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FINSRIITVS MEDVKILLTQ ENPFFRKLSS ETYSQAKDLA KGSIVLKYEP

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against NSUN2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 767 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NSUN2 antibody (ABIN2783512)

Blanco, Bandiera, Popis, Hussain, Lombard, Aleksic, Sajini, Tanna, Cortés-Garrido, Gkatza, Dietmann, Frye: "Stem cell function and stress response are controlled by protein synthesis." in: Nature, Vol. 534, Issue 7607, pp. 335-40, (2016) (PubMed).

Target Details for NSUN2

Target: NSUN2 (NOP2/Sun Domain Family, Member 2 (NSUN2))

Alternative Name: NSUN2 (NSUN2 Products)

Synonyms: D13Wsu123e antibody, Misu antibody, MISU antibody, MRT5 antibody, SAKI antibody, TRM4 antibody, RGD1311954 antibody, NOL1/NOP2/Sun domain family member 2 antibody, NOP2/Sun RNA methyltransferase family member 2 antibody, NOP2/Sun RNA methyltransferase family, member 2 antibody, NOP2/Sun RNA methyltransferase family member 2 S homeolog antibody, Nsun2 antibody, NSUN2 antibody, nsun2.S antibody

Background: Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA).Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].
Alias Symbols: FLJ20303, MISU, SAKI, TRM4
Protein Interaction Partner: SUMO2, SUMO3, UBC, SUMO1, NEDD8, TP53RK, PUS1, EFTUD1, XPO5, UGGT1, OSGEP, PANK4, THG1L, SH3GLB1, KIAA1279, UBXN7, TTLL12, ARPC2, ACTR2, ACTR3, FUBP1, TSN, SH3GL1, RPA1, RDX, RANGAP1, PPP2CB, PFDN5, PFDN1, NRD1, PPP1R12A, HARS, GTF2E1, CALU, CALR, UBD, VC
Protein Size: 767

Molecular Weight: 86 kDa

Gene ID: 54888

NCBI Accession: NM_017755, NP_060225

UniProt: Q08J23