GOT1 antibody (N-Term)

Catalog No. ABIN2783280

Product Details anti-GOT1 Antibody

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV

Predicted Reactivity: Cow: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GOT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 413 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GOT1 antibody (ABIN2783280)

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Takeda, Ishida, Takahashi, Ueda: "Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from ?-ketoglutarate for vesicular loading." in: Journal of neurochemistry, Vol. 121, Issue 2, pp. 184-96, (2012) (PubMed).

Inoue, Matsumoto, Miyoshi, Kobayashi: "Elevated liver enzymes in women with a family history of diabetes." in: Diabetes research and clinical practice, Vol. 79, Issue 3, pp. e4-7, (2008) (PubMed).

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1 (GOT1 Products)

Synonyms: ASTQTL1 antibody, GIG18 antibody, cAspAT antibody, cCAT antibody, AST antibody, ISSD antibody, NSD antibody, SD antibody, SIALIN antibody, SIASD antibody, SLD antibody, xr406 antibody, CASPAT antibody, ASPARTATE AMINOTRANSFERASE antibody, ATAAT antibody, MATERNAL EFFECT EMBRYO ARREST 17 antibody, MEE17 antibody, T26C19.9 antibody, T26C19_9 antibody, aspartate aminotransferase antibody, aatA antibody, 83.t00021 antibody, AI789014 antibody, Got-1 antibody, Aspat antibody, Gaspat antibody, AL022787 antibody, FABP-pm antibody, Got-2 antibody, mAspAT antibody, ASPATA antibody, glutamic-oxaloacetic transaminase 1 antibody, solute carrier family 17 member 5 antibody, glutamic-oxaloacetic transaminase 1 homeolog antibody, aspartate aminotransferase antibody, glutamic-oxaloacetic transaminase 1, soluble antibody, glutamic-oxaloacetic transaminase 2 antibody, glutamatic-oxaloacetic transaminase 2, mitochondrial antibody, GOT1 antibody, SLC17A5 antibody, got1.S antibody, AAT antibody, aspC antibody, EHI_006080 antibody, AST2 antibody, ast2 antibody, got1 antibody, Got1 antibody, GOT2 antibody, Got2 antibody

Background: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enz
Alias Symbols: GIG18, ASTQTL1
Protein Interaction Partner: UBC, CHRAC1, SBDS, UBE2H, TYMS, MTAP, IDH1, HPRT1, ANXA6, ABI3BP, EGFR, TMEM120A, PC,
Protein Size: 413

Molecular Weight: 46 kDa

Gene ID: 2805

NCBI Accession: NM_002079, NP_002070

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process