GOT1 antibody (N-Term)

Catalog No. ABIN2783280

Product Details anti-GOT1 Antibody

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Goat, Guinea Pig, Horse, Rabbit, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: MAPPSVFAEV PQAQPVLVFK LTADFREDPD PRKVNLGVGA YRTDDCHPWV

Predicted Reactivity: Cow: 100%, Goat: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 92%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against GOT1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GOT1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 413 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-GOT1 antibody (ABIN2783280)

Povlsen, Løfgren, Dalgas, Birkler, Johannsen, Støttrup, Bøtker: "Protection against myocardial ischemia-reperfusion injury at onset of type 2 diabetes in Zucker diabetic fatty rats is associated with altered glucose oxidation." in: PLoS ONE, Vol. 8, Issue 5, pp. e64093, (2013) (PubMed).

Takeda, Ishida, Takahashi, Ueda: "Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from ?-ketoglutarate for vesicular loading." in: Journal of neurochemistry, Vol. 121, Issue 2, pp. 184-96, (2012) (PubMed).

Inoue, Matsumoto, Miyoshi, Kobayashi: "Elevated liver enzymes in women with a family history of diabetes." in: Diabetes research and clinical practice, Vol. 79, Issue 3, pp. e4-7, (2008) (PubMed).

Target Details for GOT1

Target: GOT1 (Glutamic-Oxaloacetic Transaminase 1, Soluble (Aspartate Aminotransferase 1) (GOT1))

Alternative Name: GOT1 (GOT1 Products)

Background: Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enz
Alias Symbols: GIG18, ASTQTL1
Protein Interaction Partner: UBC, CHRAC1, SBDS, UBE2H, TYMS, MTAP, IDH1, HPRT1, ANXA6, ABI3BP, EGFR, TMEM120A, PC,
Protein Size: 413

Molecular Weight: 46 kDa

Gene ID: 2805

NCBI Accession: NM_002079, NP_002070

UniProt: P17174

Pathways: Hepatitis C, Monocarboxylic Acid Catabolic Process, Methionine Biosynthetic Process