ARF1 antibody (Middle Region)

Catalog No. ABIN2783267

Product Details anti-ARF1 Antibody

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Cow, Dog, Zebrafish (Danio rerio), Sheep, Rabbit, Guinea Pig, Horse, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Sequence: MRMLAEDELR DAVLLVFANK QDLPNAMNAA EITDKLGLHS LRHRNWYIQA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Yeast 100%

Characteristics: This is a rabbit polyclonal antibody against ARF1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ARF1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 181 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ARF1

Target: ARF1 (ADP-Ribosylation Factor 1 (ARF1))

Alternative Name: ARF1 (ARF1 Products)

Synonyms: wu:fb33e02 antibody, wu:fb78h01 antibody, arf2 antibody, arf-1 antibody, MGC52573 antibody, ADP-RIBOSYLATION FACTOR antibody, ADP-RIBOSYLATION FACTOR 1A antibody, ADP-ribosylation factor 1 antibody, ATARF antibody, ATARF1 antibody, ATARFA1A antibody, F28C11.12 antibody, ARF1 antibody, arf1 antibody, ADP ribosylation factor 1 antibody, ADP-ribosylation factor 1 antibody, ADP ribosylation factor 1 L homeolog antibody, ADP ribosylation factor 5 L homeolog antibody, ADP ribosylation factor 1 pseudogene antibody, adp-ribosylation factor 1 antibody, ARF1 antibody, Arf1 antibody, arf1 antibody, arf1.L antibody, arf5.L antibody, NCU08340 antibody, TRIADDRAFT_63238 antibody, PAAG_08805 antibody, LOC100280562 antibody, LOC100280918 antibody, LOC474591 antibody, CLUG_01013 antibody

Background: ADP-ribosylation factor 1 (ARF1) is a member of the human ARF family. The family is composed of small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. These protein, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
Alias Symbols: -
Protein Interaction Partner: GGA1, GGA3, GGA2, HUWE1, UBC, FBXO6, MMS19, TMEM106A, VCAM1, ITGA4, ATF2, ARF3, UBD, GRK5, CDK2, HERC1, Proser1, AI837181, Kif1c, Bach1, nef, COPB1, GEA1, WBP11, TMED2, PICK1, NOA1, TMED10, EEF1G, PLEKHA8, COPG1, ARFIP1, ARFIP2, ARHGAP21, ARFGAP1, AP3D1,
Protein Size: 181

Molecular Weight: 21 kDa

Gene ID: 375

NCBI Accession: NM_001658, NP_001649

UniProt: P61204

Pathways: Transition Metal Ion Homeostasis, Inositol Metabolic Process