Myosin 9 antibody (Middle Region)

Catalog No. ABIN2783216

Product Details anti-Myosin 9 Antibody

Target: Myosin 9 (MYH9)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Dog, Pig, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Myosin 9 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DAMNREVSSL KNKLRRGDLP FVVPRRMARK GAGDGSDEEV DGKADGAEAK

Predicted Reactivity: Dog: 93%, Guinea Pig: 86%, Human: 100%, Mouse: 86%, Pig: 93%, Rabbit: 86%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against MYH9. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human MYH9

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1960 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Myosin 9 antibody (ABIN2783216)

Sabbir, Dillon, Mowat: "Dlc1 interaction with non-muscle myosin heavy chain II-A (Myh9) and Rac1 activation." in: Biology open, Vol. 5, Issue 4, pp. 452-60, (2016) (PubMed).

Target Details for Myosin 9

Target: Myosin 9 (MYH9)

Alternative Name: MYH9 (MYH9 Products)

Background: MYH9 is a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause of non-syndromic sensorineural deafness autosomal dominant type 17, Epstein syndrome, Alport syndrome with macrothrombocytopenia, Sebastian syndrome, Fechtner syndrome and macrothrombocytopenia with progressive sensorineural deafness.
Alias Symbols: DFNA17, EPSTS, FTNS, MGC104539, MHA, NMHC-II-A, NMMHCA, BDPLT6, NMMHC-IIA
Protein Interaction Partner: HUWE1, UBC, TP53, SUMO2, SUMO3, IVNS1ABP, LGR4, MDM2, RPA3, RPA2, RPA1, EED, MYL6, MYH14, CDC73, OTUD6B, AKAP10, MYL12A, MYL9, TARDBP, FN1, UBD, PIK3C3, ACE, CSNK2A1, ACTB, PAN2, ITGA4, HSP90AB1, HSP90AA1, ESR1, CASP4, IQCB1, VCAM1, PPP2CA, Fcho2, TRAF3IP
Protein Size: 1960

Molecular Weight: 226 kDa

Gene ID: 4627

NCBI Accession: NM_002473, NP_002464

UniProt: P35579

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Integrin Complex