REC8 antibody (N-Term)

Catalog No. ABIN2783199

Product Details anti-REC8 Antibody

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Cow, Dog, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This REC8 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: QLQIGVIRVY SQQCQYLVED IQHILERLHR AQLQIRIDME TELPSLLLPN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 93%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against REC8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human REC8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 547 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for REC8

Target: REC8 (REC8 Homolog (Yeast) (REC8))

Alternative Name: REC8 (REC8 Products)

Synonyms: Rec8L1 antibody, mrec antibody, HR21spB antibody, REC8L1 antibody, Rec8p antibody, REC8 meiotic recombination protein antibody, REC8 homolog (yeast) antibody, REC8 antibody, Rec8 antibody

Background: REC8 is required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II.This gene encodes a member of the kleisin family of SMC (structural maintenance of chromosome) protein partners. The protein localizes to the axial elements of chromosomes during meiosis in both oocytes and spermatocytes. In the mouse, the homologous protein is a key component of the meiotic cohesion complex, which regulates sister chromatid cohesion and recombination between homologous chromosomes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene.
Alias Symbols: HR21spB, MGC950, REC8L1, Rec8p
Protein Interaction Partner: SYCP3, SMC3, SMC1A,
Protein Size: 547

Molecular Weight: 62 kDa

Gene ID: 9985

NCBI Accession: NM_005132, NP_005123

UniProt: O95072