NDE1 antibody (Middle Region)

Catalog No. ABIN2783161

Product Details anti-NDE1 Antibody

Target: NDE1

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NDE1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: AHRGPSSSLN TPGSFRRGLD DSTGGTPLTP AARISALNIV GDLLRKVGAL

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against NDE1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human NDE1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 335 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NDE1 antibody (ABIN2783161)

Burdick, Kamiya, Hodgkinson, Lencz, DeRosse, Ishizuka, Elashvili, Arai, Goldman, Sawa, Malhotra: "Elucidating the relationship between DISC1, NDEL1 and NDE1 and the risk for schizophrenia: evidence of epistasis and competitive binding." in: Human molecular genetics, Vol. 17, Issue 16, pp. 2462-73, (2008) (PubMed).

Target Details for NDE1

Target: NDE1

Alternative Name: NDE1 (NDE1 Products)

Synonyms: 2810027M15Rik antibody, AU042936 antibody, AW822251 antibody, Nude antibody, mNudE antibody, HOM-TES-87 antibody, LIS4 antibody, NDE antibody, NUDE antibody, NUDE1 antibody, fb82g01 antibody, im:7141877 antibody, wu:fb82g01 antibody, zgc:114109 antibody, nudE neurodevelopment protein 1 antibody, Nde1 antibody, NDE1 antibody, nde1 antibody

Background: NDE1 is required for centrosome duplication and formation and function of the mitotic spindle. It is essential for the development of the cerebral cortex. NDE1 may regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex
Alias Symbols: FLJ20101, HOM-TES-87, NUDE, NUDE1, LIS4
Protein Interaction Partner: FAM9B, LZTS2, USHBP1, NDEL1, ZNF398, NDE1, WAC, UBQLN1, MED4, DISC1, TFIP11, CEP250, NDC80, ARNT2, BZRAP1, MKRN3, TTC1, TRIM27, REL, HOXA2, GOLGA2, AR, YWHAQ, PAFAH1B1, ZNF365, YWHAG, TUBG1, MAPK1, CDK1,
Protein Size: 335

Molecular Weight: 38 kDa

Gene ID: 54820

NCBI Accession: NM_017668, NP_060138