FTO antibody (N-Term)
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- Target See all FTO Antibodies
- FTO (Fat Mass and Obesity-Associated (FTO))
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Binding Specificity
- N-Term
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Reactivity
- Human, Mouse, Rat, Dog, Cow, Sheep, Pig, Rabbit, Horse, Saccharomyces cerevisiae
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FTO antibody is un-conjugated
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Application
- Western Blotting (WB)
- Sequence
- MKRVQTAEER EREAKKLRLL EELEDTWLPY LTPKDDEFYQ QWQLKYPKLV
- Predicted Reactivity
- Cow: 100%, Dog: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 93%, Rat: 100%, Sheep: 100%, Yeast: 90%
- Characteristics
- This is a rabbit polyclonal antibody against Fto. It was validated on Western Blot.
- Purification
- Affinity Purified
- Immunogen
- The immunogen is a synthetic peptide corresponding to a region of Mouse
- Top Product
- Discover our top product FTO Primary Antibody
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- Application Notes
- Optimal working dilutions should be determined experimentally by the investigator.
- Comment
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Antigen size: 502 AA
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
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- Target
- FTO (Fat Mass and Obesity-Associated (FTO))
- Alternative Name
- Fto (FTO Products)
- Synonyms
- AW743446 antibody, mKIAA1752 antibody, RGD1305121 antibody, FTO, alpha-ketoglutarate dependent dioxygenase antibody, fat mass and obesity associated antibody, fat mass and obesity associated L homeolog antibody, FTO antibody, Fto antibody, fto.L antibody
- Background
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Fto is a dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Fto has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Fto has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Fto has no activity towards 1-methylguanine and no detectable activity towards double-stranded DNA. Fto requires molecular oxygen, alpha-ketoglutarate and iron. Fto contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Fto contributes to the regulation of body size and body fat accumulation.
Alias Symbols: AW743446, mKIAA1752
Protein Interaction Partner: Eed,
Protein Size: 502 - Molecular Weight
- 58 kDa
- Gene ID
- 26383
- NCBI Accession
- NM_011936, NP_036066
- UniProt
- Q8BGW1
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