AGPAT5 antibody (N-Term)

Catalog No. ABIN2782960

Product Details anti-AGPAT5 Antibody

Target: AGPAT5 (1-Acylglycerol-3-Phosphate O-Acyltransferase 5 (Lysophosphatidic Acid Acyltransferase, Epsilon) (AGPAT5))

Binding Specificity: N-Term

Reactivity: Human, Rat, Cow, Pig, Saccharomyces cerevisiae

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AGPAT5 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: RLLSAFLPAR FYQALDDRLY CVYQSMVLFF FENYTGVQIL LYGDLPKNKE

Predicted Reactivity: Cow: 86%, Human: 100%, Pig: 86%, Rat: 79%, Yeast: 77%

Characteristics: This is a rabbit polyclonal antibody against AGPAT5. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human AGPAT5

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 364 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-AGPAT5 antibody (ABIN2782960)

Agarwal, Barnes, Garg: "Functional characterization of human 1-acylglycerol-3-phosphate acyltransferase isoform 8: cloning, tissue distribution, gene structure, and enzymatic activity." in: Archives of biochemistry and biophysics, Vol. 449, Issue 1-2, pp. 64-76, (2006) (PubMed).

Target Details for AGPAT5

Target: AGPAT5 (1-Acylglycerol-3-Phosphate O-Acyltransferase 5 (Lysophosphatidic Acid Acyltransferase, Epsilon) (AGPAT5))

Alternative Name: AGPAT5 (AGPAT5 Products)

Synonyms: AGPAT5 antibody, LPAAT-e antibody, lpaate antibody, 1agpat5 antibody, DKFZp469J1022 antibody, 1AGPAT5 antibody, LPAATE antibody, RGD1306405 antibody, wu:fc35d05 antibody, zgc:154071 antibody, 1110013A05Rik antibody, D8Ertd319e antibody, 1-acylglycerol-3-phosphate O-acyltransferase 5 antibody, 1-acylglycerol-3-phosphate O-acyltransferase 5 (lysophosphatidic acid acyltransferase, epsilon) antibody, AGPAT5 antibody, agpat5 antibody, Agpat5 antibody

Background: Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Sarcomeric mitochondrial creatine kinase has 80 % homology with the coding exons of ubiquitous mitochondrial creatine kinase. This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis. Three transcript variants encoding the same protein have been found for this gene.This gene encodes a member of the 1-acylglycerol-3-phosphate O-acyltransferase family. This integral membrane protein converts lysophosphatidic acid to phosphatidic acid, the second step in de novo phospholipid biosynthesis.
Alias Symbols: 1-AGPAT5, LPAAT-e, LPAAT-epsilon, LPAATE, 1AGPAT5
Protein Interaction Partner: UBQLN1, UBC, ELAVL1, SUMO1,
Protein Size: 364

Molecular Weight: 42 kDa

Gene ID: 55326

NCBI Accession: NM_018361, NP_060831

UniProt: Q9NUQ2