KIRREL antibody (Middle Region)

Catalog No. ABIN2782952

Product Details anti-KIRREL Antibody

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Binding Specificity: Middle Region

Reactivity: Human, Rat, Mouse, Horse, Cow, Dog, Rabbit, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIRREL antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FLEVGTLERY TVERTNSGSG VLSTLTINNV MEADFQTHYN CTAWNSFGPG

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against KIRREL. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human KIRREL

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 757 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-KIRREL antibody (ABIN2782952)

Ito, Sugihara, Asaka, Toyama, Yoshihara, Furuichi, Wada, Asano: "Glycoprotein hyposialylation gives rise to a nephrotic-like syndrome that is prevented by sialic acid administration in GNE V572L point-mutant mice." in: PLoS ONE, Vol. 7, Issue 1, pp. e29873, (2012) (PubMed).

Target Details for KIRREL

Target: KIRREL (NEPH1) (Kin of IRRE Like 1 (NEPH1))

Alternative Name: KIRREL (NEPH1 Products)

Synonyms: NEPH1 antibody, 6720469N11Rik antibody, Kirrel1 antibody, Neph1 antibody, nephrin related antibody, kirre like nephrin family adhesion molecule 1 antibody, kin of IRRE like (Drosophila) antibody, Smp_104390 antibody, Smp_140170 antibody, KIRREL1 antibody, Kirrel antibody, Kirrel1 antibody

Background: KIRREL (NEPH1) is a member of the nephrin-like protein family, which includes NEPH2 and NEPH3. The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. NEPH1 is a member of the nephrin-like protein family, which includes NEPH2 (MIM 607761) and NEPH3 (MIM 607762). The cytoplasmic domains of these proteins interact with the C terminus of podocin (NPHS2, MIM 604766), and the genes are expressed in kidney podocytes, cells involved in ensuring size- and charge-selective ultrafiltration (Sellin et al., 2003 [PubMed 12424224]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-337 AY302131.1 1-337 338-3211 BC109193.1 1-2874 3212-3609 AK001707.1 1904-2301 3610-3628 BC082969.1 2273-2291
Alias Symbols: FLJ10845, MGC129542, MGC129543, NEPH1
Protein Interaction Partner: UBC, SAV1, LATS1, GRB2, KIRREL, NPHS2, NPHS1, TJP1,
Protein Size: 757

Molecular Weight: 84 kDa

Gene ID: 55243

NCBI Accession: NM_018240, NP_060710

UniProt: Q96J84

Pathways: Regulation of Actin Filament Polymerization