Anoctamin 10 antibody (C-Term)

Catalog No. ABIN2782940

Product Details anti-Anoctamin 10 Antibody

Target: Anoctamin 10 (ANO10)

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Rabbit, Zebrafish (Danio rerio), Dog, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Anoctamin 10 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: LKADIDATLY EQVILEKEMG TYLGTFDDYL ELFLQFGYVS LFSCVYPLAA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 92%

Characteristics: This is a rabbit polyclonal antibody against TMEM16K. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human TMEM16K

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 660 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Anoctamin 10 antibody (ABIN2782940)

Wanitchakool, Ousingsawat, Sirianant, Cabrita, Faria, Schreiber, Kunzelmann: "Cellular defects by deletion of ANO10 are due to deregulated local calcium signaling." in: Cellular signalling, Vol. 30, pp. 41-49, (2016) (PubMed).

Schreiber, Kunzelmann: "Expression of anoctamins in retinal pigment epithelium (RPE)." in: Pflügers Archiv : European journal of physiology, Vol. 468, Issue 11-12, pp. 1921-1929, (2016) (PubMed).

Hammer, Wanitchakool, Sirianant, Papiol, Monnheimer, Faria, Ousingsawat, Schramek, Schmitt, Margos, Michel, Kraiczy, Pawlita, Schreiber, Schulz, Fingerle, Tumani, Ehrenreich, Kunzelmann: "A Coding Variant of ANO10, Affecting Volume Regulation of Macrophages, Is Associated with Borrelia Seropositivity." in: Molecular medicine (Cambridge, Mass.), Vol. 21, pp. 26-37, (2015) (PubMed).

Tian, Schreiber, Kunzelmann: "Anoctamins are a family of Ca2+-activated Cl- channels." in: Journal of cell science, Vol. 125, Issue Pt 21, pp. 4991-8, (2013) (PubMed).

Target Details for Anoctamin 10

Target: Anoctamin 10 (ANO10)

Alternative Name: TMEM16K (ANO10 Products)

Synonyms: TMEM16K antibody, ano10 antibody, zgc:114140 antibody, tmem16k antibody, SCAR10 antibody, AI604832 antibody, Tmem16k antibody, RGD1308260 antibody, anoctamin 10 antibody, anoctamin 10a antibody, transmembrane protein 16K antibody, anoctamin 10 L homeolog antibody, ANO10 antibody, ano10 antibody, ano10a antibody, MCYG_02694 antibody, MGYG_08424 antibody, ano10.L antibody, Ano10 antibody

Background: TMEM16K is a multi-pass membrane protein. It belongs to the anoctamin family. TMEM16K may act as a calcium-activated chloride channel.
Alias Symbols: FLJ10375, MGC47890, SCAR10, TMEM16K
Protein Interaction Partner: UBC,
Protein Size: 660

Molecular Weight: 76 kDa

Gene ID: 55129

NCBI Accession: NM_018075, NP_060545

UniProt: Q9NW15