NNT antibody (N-Term)

Catalog No. ABIN2782785

Product Details anti-NNT Antibody

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NNT antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: IVRGFDTRAA ALEQFKSLGA EPLEVDLKES GEGQGGYAKE MSKEFIEAEM

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

Characteristics: This is a rabbit polyclonal antibody against NNT. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human NNT

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 1086 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-NNT antibody (ABIN2782785)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for NNT

Target: NNT (Nicotinamide Nucleotide Transhydrogenase (NNT))

Alternative Name: NNT (NNT Products)

Synonyms: Afu5g02780 antibody, GCCD4 antibody, 4930423F13Rik antibody, AI323702 antibody, BB168308 antibody, wu:fa20d10 antibody, wu:fc86a04 antibody, zgc:76979 antibody, Nicotinamide Nucleotide Transhydrogenase antibody, nicotinamide nucleotide transhydrogenase antibody, nicotinamide nucleotide transhydrogenase L homeolog antibody, nnt-1 antibody, AFUA_5G02780 antibody, nnt antibody, NNT antibody, Nnt antibody, nnt.L antibody

Background: NNT is an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. This gene encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.
Alias Symbols: MGC126502, MGC126503
Protein Interaction Partner: MVP, UBC, TIMM10, PTCD1, FKBP8, POFUT1, UQCR11, ATP6V1F, PRPF3, LAMTOR3, SF1, THY1, ABCD3, NDUFS6, NDUFA9, SLC26A2, ATP5B, SLC25A6, SIRT7, MME, HNRNPA1,
Protein Size: 1086

Molecular Weight: 114 kDa

Gene ID: 23530

NCBI Accession: NM_012343, NP_036475

UniProt: Q13423

Pathways: Proton Transport