Reticulon 4 antibody (Middle Region)

Catalog No. ABIN2782749

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Binding Specificity: Middle Region

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit, Sheep, Horse, Guinea Pig, Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: FRIYKGVIQA IQKSDEGHPF RAYLESEVAI SEELVQKYSN SALGHVNCTI

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Sheep: 100%, Zebrafish: 79%

Characteristics: This is a rabbit polyclonal antibody against RTN4. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human RTN4

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 392 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Reticulon 4 antibody (ABIN2782749)

Beetz, Koch, Khundadze, Zimmer, Nietzsche, Hertel, Huebner, Mumtaz, Schweizer, Dirren, Karle, Irintchev, Alvarez, Redies, Westermann, Kurth, Deufel, Kessels, Qualmann, Hübner: "A spastic paraplegia mouse model reveals REEP1-dependent ER shaping." in: The Journal of clinical investigation, Vol. 123, Issue 10, pp. 4273-82, (2013) (PubMed).

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4 (RTN4 Products)

Background: RTN4 belongs to the family of reticulons. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. RTN4 is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates.This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified.
Alias Symbols: ASY, NI220/250, NOGO, NOGO-A, NOGOC, NSP, NSP-CL, Nbla00271, Nbla10545, Nogo-B, Nogo-C, RTN-X, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C
Protein Interaction Partner: HUWE1, SYT16, ZFYVE21, RTN4, SNX15, ARL6IP1, SNX1, UBC, RPA3, RPA2, RPA1, vpu, BAIAP2, LAMA4, ATF2, CLN8, TMEM65, S100A16, EPPK1, SNX12, ACAA2, SLC9A3R2, TJP1, HNRNPM, ILF3, IGFBP7, DDB1, SLC25A10, CIRBP, TERF1, POT1, RPS27, NR4A1, HLA-DPB1, RTN3, RTN4IP1
Protein Size: 392

Molecular Weight: 42 kDa

Gene ID: 57142

NCBI Accession: NM_207520, NP_997403

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome