Lamin B2 antibody (N-Term)

Catalog No. ABIN2782594

Product Details anti-Lamin B2 Antibody

Target: Lamin B2 (LMNB2)

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Lamin B2 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MATPLPGRAG GPATPLSPTR LSRLQEKEEL RELNDRLAHY IDRVRALELE

Predicted Reactivity: Cow: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 85%, Rat: 92%

Characteristics: This is a rabbit polyclonal antibody against LMNB2. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human LMNB2

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 600 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-Lamin B2 antibody (ABIN2782594)

Jacinto, Benner, Hetzer: "The nucleoporin Nup153 regulates embryonic stem cell pluripotency through gene silencing." in: Genes & development, Vol. 29, Issue 12, pp. 1224-38, (2015) (PubMed).

Tsai, Wang, Heidinger, Shumaker, Adam, Goldman, Zheng: "A mitotic lamin B matrix induced by RanGTP required for spindle assembly." in: Science (New York, N.Y.), Vol. 311, Issue 5769, pp. 1887-93, (2006) (PubMed).

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Alternative Name: LMNB2 (LMNB2 Products)

Synonyms: lmnb2-a antibody, LMNB2 antibody, lmn2 antibody, lamb2 antibody, Lamin-L(II) antibody, LOC100136040 antibody, im:7142331 antibody, lamin antibody, wu:fb94e05 antibody, wu:fb95e12 antibody, wu:fc15d06 antibody, wu:fc49h03 antibody, lamin-b2 antibody, lmnb2 antibody, LAMB2 antibody, LMN2 antibody, RGD1563803 antibody, lamin B2 S homeolog antibody, lamin B2 antibody, lamin B2 L homeolog antibody, lmnb2.S antibody, LMNB2 antibody, lmnb2 antibody, lmnb2.L antibody, Lmnb2 antibody

Background: The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. LMNB2 is one of the two B type proteins, B2.The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B2. This gene is in a head-to-tail orientation with the gene for the translocase of inner mitochondrial membrane 13 homolog gene.
Alias Symbols: LAMB2, LMN2, MGC2721
Protein Interaction Partner: UBC, GPRASP2, SUZ12, EED, RNF2, LMNA, PAN2, VCP, UBD, SIRT7, ELAVL1, SVIL, BANF1, PRKCD, LMNB2, TMPO, XRCC5, ORC2,
Protein Size: 600

Molecular Weight: 68 kDa

Gene ID: 84823

NCBI Accession: NM_032737, NP_116126

UniProt: Q03252

Pathways: Apoptosis, Caspase Cascade in Apoptosis