RMI1 antibody (N-Term)

Catalog No. ABIN405814

Product Details anti-RMI1 Antibody

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Horse, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RMI1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: DGILEIPKGE LNGFYALQIN SLVDVSQPAY SQIQKLRGKN TTNDLVTAEA

Predicted Reactivity: Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 93%, Rat: 93%

Characteristics: This is a rabbit polyclonal antibody against RMI1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human RMI1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 625 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for RMI1

Target: RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1 (RMI1))

Alternative Name: RMI1 (RMI1 Products)

Background: RMI1 is an essential component of the RMI complex, a complex that plays an important role in the processing of homologous recombination intermediates to limit DNA crossover formation in cells. RMI1 promotes TOP3A binding to double Holliday junctions (DHJ) and hence stimulates TOP3A-mediated dissolution. RMI1 is required for BLM phosphorylation during mitosis. Within the BLM complex, RMI1 is required for BLM and TOP3A stability.RMI1 is a component of protein complexes that limit DNA crossover formation via the dissolution of double Holliday junctions (Raynard et al., 2006 [PubMed 16595695]).[supplied by OMIM]. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-136 BP327553.1 1-136 137-885 DN997105.1 9-757 886-2740 AL732446.4 14777-16631 2741-3508 BC039999.2 2566-3333
Alias Symbols: BLAP75, C9orf76, FLJ12888, RP11-346I8.1
Protein Interaction Partner: APITD1, STRA13, UBC, RPA3, RPA2, RPA1, TOPBP1, BLM, NR1H2, UBE3A, HSPA13, PFDN1, Ercc6l, Top3a, FANCM, RMI2, FANCF, RMI1, FANCA,
Protein Size: 625

Molecular Weight: 70 kDa

Gene ID: 80010

NCBI Accession: NM_024945, NP_079221

UniProt: Q9H9A7

Pathways: DNA Damage Repair