MRPL15 antibody (N-Term)

Catalog No. ABIN2782532

Product Details anti-MRPL15 Antibody

Target: MRPL15 (Mitochondrial Ribosomal Protein L15 (MRPL15))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Zebrafish (Danio rerio), Cow, Guinea Pig, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MRPL15 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: KPERRPRGRR RGRKCGRGHK GERQRGTRPR LGFEGGQTPF YIRIPKYGFN

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against MRPL15. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human MRPL15

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 296 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-MRPL15 antibody (ABIN2782532)

Mehrle, Rosenfelder, Schupp, del Val, Arlt, Hahne, Bechtel, Simpson, Hofmann, Hide, Glatting, Huber, Pepperkok, Poustka, Wiemann: "The LIFEdb database in 2006." in: Nucleic acids research, Vol. 34, Issue Database issue, pp. D415-8, (2005) (PubMed).

Target Details for MRPL15

Target: MRPL15 (Mitochondrial Ribosomal Protein L15 (MRPL15))

Alternative Name: MRPL15 (MRPL15 Products)

Synonyms: L15mt antibody, MRP-L15 antibody, MRP-L7 antibody, RPML7 antibody, HSPC145 antibody, Rpml7 antibody, zgc:92856 antibody, mitochondrial ribosomal protein L15 antibody, mitochondrial ribosomal protein L15 L homeolog antibody, MRPL15 antibody, Mrpl15 antibody, mrpl15 antibody, mrpl15.L antibody

Background: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL15 belongs to the ribosomal protein L15P family. It is a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75 % protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.
Alias Symbols: HSPC145, MRP-L7, RPML7, L15mt, MRP-L15
Protein Interaction Partner: CCNDBP1, RUNDC3A, UBC, SOX2, MRPL55, MRPL10, MRPL45, TMEM177, MRPL57, MRPL9, MRPL11, MRPL32, MRPL38, MRPL40, MRPL14, MARC1, NDUFA12, MRPL16, MRPL50, MRPL39, MRPL4, MRPL13, FKBP8, SCFD1, MRPL19, EIF3C, MRPL12, OGDH, NDUFS4, ILF3, DLST, CYC1, COX15, CAND1,
Protein Size: 296

Molecular Weight: 33 kDa

Gene ID: 29088

NCBI Accession: NM_014175, NP_054894

UniProt: Q9P015