CTH antibody (N-Term)

Catalog No. ABIN2782463

Product Details anti-CTH Antibody

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Zebrafish (Danio rerio), Guinea Pig, Horse, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CTH antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: VPPISLSTTF KQGAPGQHSG FEYSRSGNPT RNCLEKAVAA LDGAKYCLAF

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 93%, Horse: 92%, Human: 100%, Mouse: 100%, Pig: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against CTH. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human CTH

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 405 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CTH antibody (ABIN2782463)

Perry, Kumai, Porteus, Tzaneva, Kwong: "An emerging role for gasotransmitters in the control of breathing and ionic regulation in fish." in: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 186, Issue 2, pp. 145-59, (2016) (PubMed).

Kumai, Porteus, Kwong, Perry: "Hydrogen sulfide inhibits Na+ uptake in larval zebrafish, Danio rerio." in: Pflügers Archiv : European journal of physiology, Vol. 467, Issue 4, pp. 651-64, (2015) (PubMed).

Porteus, Abdallah, Pollack, Kumai, Kwong, Yew, Milsom, Perry: "The role of hydrogen sulphide in the control of breathing in hypoxic zebrafish (Danio rerio)." in: The Journal of physiology, Vol. 592, Issue 14, pp. 3075-88, (2014) (PubMed).

Target Details for CTH

Target: CTH (Cystathionase (Cystathionine gamma-Lyase) (CTH))

Alternative Name: CTH (CTH Products)

Background: CTH is a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in its gene cause cystathioninuria.This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Alias Symbols: MGC9471
Protein Interaction Partner: GUCD1, WDYHV1, RECK, CTH, PTMA, GMDS, CAPN2, ATIC, SDCBP2, UBC, ELAVL1,
Protein Size: 405

Molecular Weight: 44 kDa

Gene ID: 1491

NCBI Accession: NM_001902, NP_001893

UniProt: P32929

Pathways: ER-Nucleus Signaling, Warburg Effect